Ohtsuka H, Yajima H, Maruyama K, Kimura S
Department of Biology, Faculty of Science, Chiba University, Japan.
FEBS Lett. 1997 Jan 13;401(1):65-7. doi: 10.1016/s0014-5793(96)01432-9.
Connectin/titin is a 3000 kDa protein which links the myosin filament to the Z-line in vertebrate striated muscle sarcomeres. To search for the Z-line proteins to which connectin binds, the yeast two-hybrid system was applied using cDNA coding the N-terminal 63 kDa fragment of connectin. Two clones coding the C-terminal half region of alpha-actinin (amino acids, 343-897 and 446-897) were obtained. Enzyme-linked immunosorbent assay clearly demonstrated the interactions of alpha-actinin and the N-terminal 63 kDa fragment of connectin in vitro. Thus it is concluded that the N-terminal 63 kDa portion of connectin binds to alpha-actinin in the Z-line of myofibrillar sarcomeres.
伴肌动蛋白/肌联蛋白是一种分子量为3000 kDa的蛋白质，它将肌球蛋白丝与脊椎动物横纹肌肌节中的Z线相连。为了寻找与伴肌动蛋白结合的Z线蛋白，利用编码伴肌动蛋白N端63 kDa片段的cDNA应用酵母双杂交系统。获得了两个编码α-辅肌动蛋白C端半区（氨基酸343 - 897和446 - 897）的克隆。酶联免疫吸附测定清楚地证明了α-辅肌动蛋白与伴肌动蛋白N端63 kDa片段在体外的相互作用。因此得出结论，伴肌动蛋白的N端63 kDa部分与肌原纤维肌节Z线中的α-辅肌动蛋白结合。
