Sakaguchi K, Nagayama M, Masaoka T, Nishimura A, Kageyama K, Shirai M, Akahori F
Department of Environmental Health, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Vet Hum Toxicol. 1997 Feb;39(1):1-5.
The serum cholinesterase (ChE) isoenzyme patterns consist of 5 bands for normal beagle dogs. We examined the ChE isoenzyme patterns and ChE activities of 4 groups, each of which consisted of 4 dogs, given the organophosphate (OP) compounds fenthion (P = S type), isoxathion (P = S type), dichlorvos (P = O type) or propaphos (P = O type) po at 220, 75, 120 or 90 mg/kg respectively. Blood samples were collected for 30 d after the OP administrations. ChE isoenzyme bands 4 and 5 were inhibited 12 h after fenthion and isoxathion administration; dichlorvos and propaphos produced suppression of main bands 4 and 5 20 min after administration. This change was effective to differentiate the type of OP administered (P = S and P = O), while the main ChE isoenzyme bands of dog serum reflected inhibition of serum ChE activity.
正常比格犬的血清胆碱酯酶（ChE）同工酶谱由5条带组成。我们检测了4组犬（每组4只）的ChE同工酶谱和ChE活性，这4组犬分别经口给予有机磷（OP）化合物倍硫磷（P = S型）、异恶唑磷（P = S型）、敌敌畏（P = O型）或丙虫磷（P = O型），剂量分别为220、75、120或90 mg/kg。在给予OP化合物后30天内采集血样。给予倍硫磷和异恶唑磷后12小时，ChE同工酶带4和5受到抑制；给予敌敌畏和丙虫磷后20分钟，主要条带4和5受到抑制。这种变化有助于区分所给予的OP类型（P = S型和P = O型），而犬血清中的主要ChE同工酶带反映了血清ChE活性的抑制情况。
