Yamagaki T, Ishizuka Y, Kawabata S, Nakanishi H
Department of Biochemistry, Saitama University, Japan.
Rapid Commun Mass Spectrom. 1996;10(15):1887-90. doi: 10.1002/(SICI)1097-0231(199612)10:15<1887::AID-RCM773>3.0.CO;2-2.
gamma-Cyclodextrin, maltosyl-alpha-cyclodextrin and diglucosyl-alpha-cyclodextrin were analyzed using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. All of these compounds have the same molecular weight (M.W. = 1297.15) and consist of only D-glucopyranose. From a comparison of the intensities in the post-source decay (PSD) fragment spectra of these cyclodextrin derivatives, correlation between the chemical structures and the relative intensities in the PSD fragment ions was found. The correlation is considered to be caused by the difference in the number of cleavage sites at the glycosyl binding. It was found that the intensity of the PSD ion resulting from one cleavage is higher than that resulting from two cleavages at a glycosyl bond. The results show that PSD fragment-ion spectrum method used in MALDI-TOF mass spectrometry is a very powerful technique for the structural analyses of the sugar-substituted cyclodextrins.
使用基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱法对γ-环糊精、麦芽基-α-环糊精和二葡糖基-α-环糊精进行了分析。所有这些化合物具有相同的分子量（M.W. = 1297.15），且仅由D-吡喃葡萄糖组成。通过比较这些环糊精衍生物的源后衰变（PSD）碎片谱中的强度，发现了化学结构与PSD碎片离子相对强度之间的相关性。这种相关性被认为是由糖基结合处裂解位点数量的差异引起的。结果发现，在糖基键处一次裂解产生的PSD离子强度高于两次裂解产生的PSD离子强度。结果表明，MALDI-TOF质谱法中使用的PSD碎片离子谱方法是用于糖基取代环糊精结构分析的非常强大的技术。
