Krause J, Stoeckli M, Schlunegger U P
Institute of Organic Chemistry, University of Berne, Switzerland.
Rapid Commun Mass Spectrom. 1996;10(15):1927-33. doi: 10.1002/(SICI)1097-0231(199612)10:15<1927::AID-RCM709>3.0.CO;2-V.
A new group of compounds has been successfully tested as matrices for ultraviolet matrix-assisted laser desorption/ionization mass spectrometry (UV-MALDI MS). Several new matrices for UV-MALDI MS have been found by choosing, as potential matrices, compounds that perform an intramolecular proton transfer along an intramolecular H-bond under UV irradiation. Compounds of this type are, for example, salicylamide, salicylanilide, several ortho-hydroxyacetophenones and ortho-hydroxybenzophenones. The matrix activity of these compounds is compared to the corresponding meta- and para-isomers and to the matrix activity of such well known matrices as 2,5-dihydroxybenzoic acid and 2,4,6-trihydroxyacetophenone. It was found that meta- and para-substituted hydroxycarbonyl compounds show either a significantly lower or no matrix activity compared with the ortho isomers.
一组新的化合物已成功作为紫外基质辅助激光解吸/电离质谱（UV-MALDI MS）的基质进行了测试。通过选择在紫外辐射下沿分子内氢键进行分子内质子转移的化合物作为潜在基质，发现了几种新的UV-MALDI MS基质。这类化合物例如有水杨酰胺、水杨酰苯胺、几种邻羟基苯乙酮和邻羟基二苯甲酮。将这些化合物的基质活性与相应的间位和对位异构体以及与2,5-二羟基苯甲酸和2,4,6-三羟基苯乙酮等知名基质的基质活性进行了比较。结果发现，与邻位异构体相比，间位和对位取代的羟基羰基化合物的基质活性显著较低或没有基质活性。
