Millerioux L, Brault M, Gualano V, Mignot A
Cephac Research Centre, Saint-Benoît, France.
J Chromatogr A. 1996 Apr 5;729(1-2):309-14. doi: 10.1016/0021-9673(95)00944-2.
A reversed-phase isocratic HPLC method is described for the determination of baclofen in human plasma. Solid-phase extraction using a SCX Bond Elut column is used followed by derivatization with o-phthalaldehyde-tert.-butanethiol and electrochemical detection. Both the within- and between-day R.S.D. and inaccuracy are less than 10% and 7%, respectively, even at the limit of quantification of the method, i.e., 10 ng/ml. The method was shown to give optimum performance in terms of sensitivity, precision and accuracy for the pharmacokinetic study of baclofen after a single oral administration to volunteers.
描述了一种反相等度高效液相色谱法，用于测定人血浆中的巴氯芬。采用SCX Bond Elut柱进行固相萃取，然后用邻苯二甲醛-叔丁硫醇衍生化并进行电化学检测。即使在该方法的定量限（即10 ng/ml）下，日内和日间相对标准偏差（R.S.D.）及不准确度分别小于10%和7%。该方法在对志愿者单次口服给药后巴氯芬的药代动力学研究中，在灵敏度、精密度和准确度方面均表现出最佳性能。
