新月柄杆菌中木糖依赖性启动子的分离与鉴定
Isolation and characterization of a xylose-dependent promoter from Caulobacter crescentus.
作者信息
Meisenzahl A C, Shapiro L, Jenal U
机构信息
Department of Developmental Biology, Stanford University School of Medicine, California 94305-5427, USA.
出版信息
J Bacteriol. 1997 Feb;179(3):592-600. doi: 10.1128/jb.179.3.592-600.1997.
Abstract
An inducible promoter is a useful tool for the controlled expression of a given gene. Accordingly, we identified, cloned, and sequenced a chromosomal locus, xylX, from Caulobacter crescentus which is required for growth on xylose as the sole carbon source and showed that transcription from a single site is dependent on the presence of xylose in the growth medium. P(xylX) promoter activity was determined as a function of the composition of the growth medium both in single copy and on a plasmid using different reporter genes. One hundred micromolar exogenously added xylose was required for maximal induction of P(xylX) in a strain that is unable to metabolize xylose. P(xylX) activity was induced immediately after the addition of xylose and repressed almost completely when xylose was removed from the growth medium. In addition to the strong transcriptional control, the expression of xylX is also regulated on the translational level.
摘要
可诱导启动子是用于控制特定基因表达的有用工具。因此，我们从新月柄杆菌中鉴定、克隆并测序了一个染色体位点xylX，它是利用木糖作为唯一碳源生长所必需的，并表明从单个位点的转录依赖于生长培养基中木糖的存在。使用不同的报告基因，在单拷贝和质粒上测定了P(xylX)启动子活性与生长培养基组成的函数关系。在不能代谢木糖的菌株中，需要100微摩尔外源添加的木糖才能使P(xylX)达到最大诱导。加入木糖后，P(xylX)活性立即被诱导，当从生长培养基中去除木糖时，其活性几乎完全被抑制。除了强大的转录控制外，xylX 的表达在翻译水平上也受到调控。
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