Arnberg N, Mei Y f, Wadell G
Department of Virology, University of Umeâ, Umeâ, Sweden.
Virology. 1997 Jan 6;227(1):239-44. doi: 10.1006/viro.1996.8269.
We have characterized the fibergenes of adenovirus type 19p (Ad19p), Ad19a, and Ad37 by sequencing. The fiber genes of Ad19a and Ad37 are identical and only five amino acids differ comparing Ad19a/Ad37 with Ad19p. Based on the translated sequences we calculated the isoelectrical points (Ips) and found that the fiber knobs of Ad19p, Ad19a, and Ad37 together with Ad8 display the highest Ips of all so far characterized. Two regions within the fiber knob with unusually basic characteristics have been identified. Sequence alignments revealed that the corresponding regions in other fiber knobs are highly antigenic in pepscan analysis and of importance for hemagglutination. Only two positions differ in the knobs comparing Ad19a/Ad37 with Ad19p. Hence, either of these or both amino acid residues should be expected to be responsible for the observed differences in hemagglutination between Ad19p and Ad19a/Ad37. Moreover, we have found two amino acids (Ala227 and Lys252) that are unique in their respective position in Ad19p, Ad19a, Ad37, and Ad8. Three amino acids (Lys236, Lys240, and Asn251) are unique in their respective position in Ad19a and Ad37, that manifest a tropism for the genital tract. All five amino acids colocalize within one of the two basic regions.
我们通过测序对19p型腺病毒（Ad19p）、Ad19a和Ad37的纤维基因进行了特征分析。Ad19a和Ad37的纤维基因相同，与Ad19p相比只有五个氨基酸不同。根据翻译后的序列，我们计算了等电点（Ips），发现Ad19p、Ad19a和Ad37的纤维钮与Ad8一起在所有迄今已表征的病毒中显示出最高的Ips。在纤维钮内已鉴定出两个具有异常碱性特征的区域。序列比对显示，在肽扫描分析中，其他纤维钮中的相应区域具有高度抗原性，并且对血凝作用很重要。与Ad19p相比，Ad19a/Ad37的钮中只有两个位置不同。因此，预期这些氨基酸残基中的一个或两个应负责Ad19p与Ad19a/Ad37之间观察到的血凝差异。此外，我们发现了两个氨基酸（Ala227和Lys252），它们在Ad19p、Ad19a、Ad37和Ad8的各自位置上是独特的。三个氨基酸（Lys236、Lys240和Asn251）在Ad19a和Ad37的各自位置上是独特的，它们表现出对生殖道的嗜性。所有五个氨基酸都共定位于两个碱性区域之一内。
