Huang S, Reddy V, Dasgupta N, Nemerow G R
Departments of Immunology and Molecular Biology, The Scripps Research Institute, La Jolla, California, USA.
J Virol. 1999 Apr;73(4):2798-802. doi: 10.1128/JVI.73.4.2798-2802.1999.
A 46-kDa receptor, coxsackievirus-adenovirus (Ad) receptor (CAR), mediates cell attachment of a number of different Ad serotypes; however, not all Ad serotypes utilize this receptor for infection. Moreover, the precise amino acid sequences in the Ad fiber protein that mediate cell attachment have yet to be identified. We investigated the interaction of subgroup D Ads with human ocular cells. Ad serotype 37 (Ad37), a virus associated with epidemic keratoconjunctivitis, but not a closely related virus serotype, Ad19p, exhibited preferential binding to and infection of human conjunctival cells. A single amino acid substitution in the Ad19p fiber distal domain (knob), Glu240 to Lys, conferred binding to conjunctival cells, while the reverse substitution in the Ad37 fiber abrogated cell binding. These findings provide new information on the fiber sequences that regulate Ad host cell tropism.
一种46千道尔顿的受体，柯萨奇病毒 - 腺病毒（Ad）受体（CAR），介导多种不同腺病毒血清型的细胞附着；然而，并非所有腺病毒血清型都利用该受体进行感染。此外，介导细胞附着的腺病毒纤维蛋白中精确的氨基酸序列尚未确定。我们研究了D亚组腺病毒与人眼细胞的相互作用。腺病毒血清型37（Ad37）是一种与流行性角结膜炎相关的病毒，但与其密切相关的病毒血清型Ad19p不同，它表现出对人结膜细胞的优先结合和感染。Ad19p纤维远端结构域（球形结构）中的单个氨基酸取代，即谷氨酸240突变为赖氨酸，赋予了与结膜细胞的结合能力，而Ad37纤维中的反向取代则消除了细胞结合。这些发现为调节腺病毒宿主细胞嗜性的纤维序列提供了新信息。
