Krasteva M, Garrigue J L, Horrand F, Tchou I, Descotes J, Nicolas J F
INSERM U.80, Université Claude Bernard Lyon I, Faculté de Médecine Lyon, France.
Contact Dermatitis. 1996 Nov;35(5):279-82. doi: 10.1111/j.1600-0536.1996.tb02388.x.
Contact dermatitis is a cutaneous inflammatory reaction mediated by hapten-specific T cells. We used a murine model to investigate the contact sensitivity reaction elicited by different concentrations (optimal and suboptimal) of the haptens DNFB and oxazolone applied singly or in combination. The simultaneous application of DNFB and oxazolone at optimal concentrations (0.2% and 0.4% respectively) did not significantly increase the ear swelling response induced by each of the allergens when applied singly. No contact sensitivity response was observed when the haptens were tested individually at subthreshold concentrations (0.05% and 0.1% respectively). However, mixing the 2 molecules at the same concentrations gave rise to a clinical contact sensitivity reaction. The simultaneous application of the haptens at a 2 x higher, but still suboptimal concentrations (0.1% and 0.2% respectively), elicited an inflammatory response that was significantly greater than the responses elicited by either of the haptens when applied separately. These results suggest that a "false-positive" reaction to a mix may reveal a genuine sensitization to the constituents.
接触性皮炎是一种由半抗原特异性T细胞介导的皮肤炎症反应。我们使用小鼠模型来研究单独或联合应用不同浓度（最佳浓度和次最佳浓度）的半抗原二硝基氟苯（DNFB）和恶唑酮引发的接触敏感性反应。当单独应用时，以最佳浓度（分别为0.2%和0.4%）同时应用DNFB和恶唑酮，并不会显著增加由每种变应原单独诱导的耳部肿胀反应。当对半抗原分别以阈下浓度（分别为0.05%和0.1%）进行测试时，未观察到接触敏感性反应。然而，将这两种分子以相同浓度混合会引发临床接触敏感性反应。以高出2倍但仍为次最佳浓度（分别为0.1%和0.2%）同时应用半抗原，引发的炎症反应显著大于单独应用任一一种半抗原所引发的反应。这些结果表明，对混合物的“假阳性”反应可能揭示对其成分的真正致敏。
