Otani S, Connor J A
Roche Institute of Molecular Biology, Nutley, NJ 07110, USA.
Eur J Pharmacol. 1996 Dec 27;318(1):R5-6. doi: 10.1016/s0014-2999(96)00874-6.
Homosynaptic long-term depression was induced in area CA1 of adult hippocampus by prolonged low-frequency stimulation (900 pulses at 2 Hz) in the presence of the GABAA receptor antagonist picrotoxin. Using ratio imaging with fura-2, we demonstrate that the induction of this long-term depression is associated with a rapid and transient (approximately 30 s) dendritic Ca2+ increase (approximately 500 nM) dependent on the activation of voltage-gated Ca2+ channels. This transient increase, by itself, was insufficient for long-term depression induction.
在存在GABAA受体拮抗剂荷包牡丹碱的情况下，通过长时间低频刺激（2赫兹下900个脉冲）在成年海马体的CA1区诱导出同突触长时程抑制。使用fura-2进行比率成像，我们证明这种长时程抑制的诱导与依赖电压门控Ca2+通道激活的快速且短暂（约30秒）的树突状Ca2+增加（约500 nM）有关。这种短暂增加本身不足以诱导长时程抑制。
