Tang Jun, Guo Mao, Fu Jing, Ouyang Hongjia, Tian Yunbo, Shen Xu, Huang Yunmao
College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, P.R. China.
Guangdong Provincial Key Laboratory of Waterfowl Healthy Breeding, Guangzhou, Guangdong, 510225, P.R. China.
Arch Anim Breed. 2021 Jul 22;64(2):315-323. doi: 10.5194/aab-64-315-2021. eCollection 2021.
Insulin-like growth factor 1 () is one of the endocrine hormones that plays an important role in regulating growth and development of animals. In this study, polymorphism in the 5 UTR and 3 UTR coding region and of the gene was detected by DNA sequencing technology, and the abundance of mRNA in various tissues at three growth stages of the Shitou goose was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Moreover, the differential expression of in various tissues between the Shitou goose and Wuzong goose was revealed. Two single nucleotide polymorphisms (SNPs) were found in the exon3 region of in the Shitou goose. mRNA was extensively expressed in various tissues of Shitou geese with high abundant expression in the liver, breast muscle and leg muscle at three growth stages. mRNA expression showed a trend of first increase and then decrease in the pituitary, liver, subcutaneous fat and abdominal fat tissues, but it decreased in the breast muscle and leg muscle of a Shitou goose with growing age. Expression of in the liver, leg muscle and pituitary tissues of the Shitou goose was significantly higher than that of the Wuzong goose. This provides a foundation for further study of regulatory mechanisms of in the growth and development of geese.
胰岛素样生长因子1（IGF-1）是在调节动物生长发育中起重要作用的内分泌激素之一。在本研究中，采用DNA测序技术检测IGF-1基因5′非翻译区（UTR）、3′UTR编码区的多态性，并通过实时荧光定量聚合酶链反应（qRT-PCR）测定了石鹅三个生长阶段各组织中IGF-1 mRNA的丰度。此外，还揭示了石鹅与五龙鹅各组织中IGF-1的差异表达情况。在石鹅IGF-1基因外显子3区域发现了两个单核苷酸多态性（SNP）。IGF-1 mRNA在石鹅各组织中广泛表达，在三个生长阶段的肝脏、胸肌和腿肌中表达量较高。在垂体、肝脏、皮下脂肪和腹部脂肪组织中，IGF-1 mRNA表达呈先升高后降低的趋势，但在石鹅的胸肌和腿肌中，随着日龄的增长而降低。石鹅肝脏、腿肌和垂体组织中IGF-1的表达显著高于五龙鹅。这为进一步研究IGF-1在鹅生长发育中的调控机制奠定了基础。
