Mou K, Adamson C L, Davis R L
Department of Biological Sciences, Rutgers University, Piscataway, NJ 08855, USA.
Hear Res. 1997 Jan;103(1-2):47-62. doi: 10.1016/s0378-5955(96)00163-3.
Explants of neonatal murine stria vascularis were maintained in vitro to evaluate the process of morphogenesis in cochlear tissue. Immunohistochemical and electron microscopic studies showed that the relatively undifferentiated cells in culture attained morphological features characteristic of the stria vascularis cell types in vivo (marginal, intermediate and basal cells). The three kinds of cells formed a trilaminated tissue, with the epithelial cells bordering the culture medium, basal-like cells resting on the culture substrate, and the melanocytes layered between. Furthermore, approximately 20% of these cultures displayed a unique alignment of melanocytes which formed elongated bands along the contour of the tissue edge. However, only limited cell extensions were formed between different cell types and interdigitation amongst these processes was abbreviated. Thus, cells from different embryological origins divided, migrated and reestablished appropriate cell-to-cell associations to form a layered tissue similar to the stria vascularis in vivo.
将新生小鼠血管纹的外植体进行体外培养，以评估耳蜗组织中的形态发生过程。免疫组织化学和电子显微镜研究表明，培养物中相对未分化的细胞获得了体内血管纹细胞类型（边缘细胞、中间细胞和基底细胞）的特征性形态特征。这三种细胞形成了一个三层组织，上皮细胞与培养基相邻，基底样细胞位于培养底物上，黑素细胞夹在中间。此外，约20%的这些培养物显示出黑素细胞的独特排列，它们沿着组织边缘的轮廓形成细长条带。然而，不同细胞类型之间仅形成有限的细胞延伸，并且这些突起之间的相互交错缩短。因此，来自不同胚胎学起源的细胞分裂、迁移并重新建立适当的细胞间联系，以形成类似于体内血管纹的分层组织。
