Bornstein S R, Ehrhart-Bornstein M, González-Hernández J, Schröder S, Scherbaum W A
Medizinische Klinik und Poliklinik III, Universität Leipzig, Germany.
J Endocrinol Invest. 1996 Nov;19(10):693-8. doi: 10.1007/BF03349041.
Cytokines and in particular interleukin 1 (IL-1) play a role in the micro-environment of various tumors. In addition, it is well established that IL-1 is a neurotrophic, angiogenetic and fibrogenetic factor. Using in situ hybridization we analyzed the expression of endogenous IL-1 in human pheochromocytoma (PCC). With the help of specific antibodies to B- and T-cells, macrophages, and neuroendocrine cell antigens, we characterized the distribution and localization of various cell types in human PCC. The combination of immunohistochemistry and in situ hybridization was eminently suited to defining the exact cellular source of IL-1 expression. In situ hybridization and immunostaining revealed that IL-1 mRNA was located primarily in the tumor cell itself. IL-1 may therefore constitute an important paracrine/autocrine factor in neuroendocrine tumors.
细胞因子，尤其是白细胞介素1（IL-1），在各种肿瘤的微环境中发挥作用。此外，IL-1作为一种神经营养、血管生成和纤维生成因子已得到充分证实。我们采用原位杂交技术分析了人嗜铬细胞瘤（PCC）中内源性IL-1的表达。借助针对B细胞、T细胞、巨噬细胞和神经内分泌细胞抗原的特异性抗体，我们对人PCC中各种细胞类型的分布和定位进行了表征。免疫组织化学和原位杂交相结合非常适合确定IL-1表达的确切细胞来源。原位杂交和免疫染色显示，IL-1 mRNA主要位于肿瘤细胞本身。因此，IL-1可能是神经内分泌肿瘤中一种重要的旁分泌/自分泌因子。
