Yanagihara N, Minami K, Shirakawa F, Uezono Y, Kobayashi H, Eto S, Izumi F
Department of Pharmacology, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.
Biochem Biophys Res Commun. 1994 Jan 14;198(1):81-7. doi: 10.1006/bbrc.1994.1012.
We investigated the effect of recombinant human interleukin-1 beta (IL-1 beta) on catecholamine secretion from cultured bovine adrenal medullary cells. Treatment of cultured cells with IL-1 beta (10 ng/ml) for 24 hr caused an increase in accumulation of catecholamines in the cultured medium. The accumulation of catecholamines stimulated by IL-1 beta was observed in time (4-48 hr)- and concentration (3-30 ng/ml)-dependent manners. The stimulatory effect of IL-1 beta (10 ng/ml) was completely inhibited by recombinant human IL-1 receptor antagonist (1 microgram/ml). IL-1 beta had little effect on [3H]norepinephrine uptake to cultured cells. These results suggest that IL-1 beta stimulates catecholamine secretion through activation of IL-1 receptors in adrenal medullary cells.
我们研究了重组人白细胞介素-1β（IL-1β）对培养的牛肾上腺髓质细胞儿茶酚胺分泌的影响。用IL-1β（10 ng/ml）处理培养的细胞24小时导致培养基中儿茶酚胺积累增加。观察到IL-1β刺激的儿茶酚胺积累呈时间（4 - 48小时）和浓度（3 - 30 ng/ml）依赖性方式。重组人IL-1受体拮抗剂（1微克/ml）完全抑制了IL-1β（10 ng/ml）的刺激作用。IL-1β对培养细胞摄取[3H]去甲肾上腺素的影响很小。这些结果表明，IL-1β通过激活肾上腺髓质细胞中的IL-1受体来刺激儿茶酚胺分泌。
