Sahlin S, Glauman H, Danielsson A, Einarsson K
Department of Surgery, Danderyd Hospital, Sweden.
J Hepatol. 1996 Dec;25(6):895-9. doi: 10.1016/s0168-8278(96)80294-7.
BACKGROUND/AIMS: Gallstone patients have a reduced cellular lysosome content in the gallbladder mucosa cells compared with gallstone-free subjects. The purpose of the study was to further evaluate the possible role of lysosomes in the pathogenesis of cholesterol gallstone formation in humans.
Lysosomal enzyme activities were assayed in gallbladder mucosa and for comparison in liver specimens of 19 gallstone-free subjects and 24 gallstone patients undergoing cholecystectomy.
Gallstone patients had 25-50% lower activities of the lysosomal proteases cathepsin B, D and L in their gallbladder mucosa compared with gallstone-free subjects. The activity of acid phosphatase also tended to be decreased in gallstone patients. The liver lysosomal enzyme activities were not significantly different between the two groups.
The results show that gallstone patients have diminished lysosomal enzyme activities in the gallbladder mucosa, a finding which may be related to decreased intracellular degradation of proteins and/or mucin in the mucosal cells. This may lead to a higher concentration of mucin in gallbladder bile and thus an increased risk of precipitation of cholesterol crystals and gallstone formation.
背景/目的：与无胆结石者相比，胆结石患者胆囊黏膜细胞中的细胞溶酶体含量减少。本研究的目的是进一步评估溶酶体在人类胆固醇胆结石形成发病机制中的可能作用。
对19例无胆结石者和24例接受胆囊切除术的胆结石患者的胆囊黏膜进行溶酶体酶活性检测，并以肝脏标本作为对照。
与无胆结石者相比，胆结石患者胆囊黏膜中溶酶体蛋白酶组织蛋白酶B、D和L的活性降低了25%-50%。胆结石患者的酸性磷酸酶活性也有降低趋势。两组肝脏溶酶体酶活性无显著差异。
结果表明，胆结石患者胆囊黏膜中的溶酶体酶活性降低，这一发现可能与黏膜细胞内蛋白质和/或黏蛋白降解减少有关。这可能导致胆囊胆汁中黏蛋白浓度升高，从而增加胆固醇晶体沉淀和胆结石形成的风险。
