Maar T E, Rønn L C, Bock E, Berezin V, Moran J, Pasantes-Morales H, Schousboe A
Center for Medical Biotechnology, Protein Laboratory, Panum Institute, University of Copenhagen, Denmark.
J Neurosci Res. 1997 Jan 15;47(2):163-72.
Microwell cultures of dissociated tissue from prenatal rat hippocampus and cerebral cortex as well as from early postnatal cerebellum were used for quantification of neuronal aggregation, process extension, and fasciculation. It was shown that the cells in culture from these different brain regions developed differently with regard to both architecture and rate of differentiation. The effect of a polyclonal antibody against the neural cell adhesion molecule (NCAM), the excitatory amino acid receptor agonist N-methyl-D-aspartate (NMDA), and the neurotoxin acrylamide on aggregation and fiber formation was investigated. Exposure to the NCAM antibody led to formation of fewer but larger aggregates and stimulated the morphological development of the cultures. Acrylamide affected aggregate formation, leading to smaller but more numerous aggregates, and it inhibited process extension and fasciculation. Treatment with NMDA affected process formation and led to formation of more numerous but smaller aggregates. Some of these effects were strongly tissue-dependent. Thus, large differences were seen regarding the effect of the NCAM antibody on aggregation and process extension in cultures from the different brain areas. The culture systems appear to represent convenient and reliable screening tools to study the influence of putative morphoregulatory substances on cell-cell interactions during early neuronal development.
来自产前大鼠海马体和大脑皮层以及产后早期小脑的解离组织的微孔培养物被用于定量神经元聚集、突起延伸和束状化。结果表明，来自这些不同脑区的培养细胞在结构和分化速率方面表现出不同的发育情况。研究了抗神经细胞粘附分子（NCAM）的多克隆抗体、兴奋性氨基酸受体激动剂N-甲基-D-天冬氨酸（NMDA）和神经毒素丙烯酰胺对聚集和纤维形成的影响。暴露于NCAM抗体导致形成的聚集体数量减少但体积增大，并刺激了培养物的形态发育。丙烯酰胺影响聚集体形成，导致聚集体更小但数量更多，并抑制突起延伸和束状化。用NMDA处理影响突起形成，并导致形成更多但更小的聚集体。其中一些效应强烈依赖于组织。因此，在来自不同脑区的培养物中，NCAM抗体对聚集和突起延伸的影响存在很大差异。这些培养系统似乎是研究假定的形态调节物质对早期神经元发育过程中细胞间相互作用影响的方便且可靠的筛选工具。
