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微小隐孢子虫的分子核型分析：八条染色体和一种低分子量分子的证据

Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosomes and a low-molecular-size molecule.

作者信息

Blunt D S, Khramtsov N V, Upton S J, Montelone B A

机构信息

Division of Biology, Kansas State University, Manhattan 66506-4901, USA.

出版信息

Clin Diagn Lab Immunol. 1997 Jan;4(1):11-3. doi: 10.1128/cdli.4.1.11-13.1997.

DOI:10.1128/cdli.4.1.11-13.1997
PMID:9008274
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC170468/
Abstract

We report improved separation of chromosome-sized DNA molecules of the coccidian parasite Cryptosporidium parvum with contour-clamped homogeneous electric fields (CHEF). We used scanning densitometry to determine that the most likely number of chromosomes is eight. Molecular probes consisting of cloned genes were used to distinguish each of five bands visible on CHEF gels. We have also identified a low-molecular-size DNA molecule possibly related to the 35-kb circular DNAs found in other Apicomplexa.

摘要

我们报告了利用轮廓夹恒定电场（CHEF）改进了对球虫寄生虫微小隐孢子虫染色体大小的DNA分子的分离。我们使用扫描光密度测定法确定最可能的染色体数目为八条。由克隆基因组成的分子探针被用于区分在CHEF凝胶上可见的五条带中的每一条。我们还鉴定出一种低分子大小的DNA分子，它可能与在其他顶复门生物中发现的35 kb环状DNA有关。

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