转化生长因子β-1在内毒素诱导性葡萄膜炎中的作用。

Effect of transforming growth factor beta-1 in endotoxin-induced uveitis.

作者信息

Peng B, Li Q, Roberge F G, Chan C C

机构信息

Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, Maryland 20892-1858, USA.

出版信息

Invest Ophthalmol Vis Sci. 1997 Jan;38(1):257-60.

PMID:9008650

Abstract

PURPOSE

Transforming growth factor beta-1 (TGF beta-1) can modulate inflammation. Endotoxin-induced uveitis (EIU) is characterized by acute ocular inflammation related to the release of cytokines, including interleukin (IL)-6. The authors investigated the effect of TGF beta-1 on EIU in mice.

METHODS

Three independent experiments were performed. Endotoxin-induced uveitis was induced in C3H/HeN mice by an injection of 200 micrograms of lipopolysaccharide (LPS). Two micrograms of TGF beta-1 in 0.1 ml phosphate-buffered saline (PBS) or 0.1 ml PBS alone was administered intraperitoneally at 8 hours after LPS injection. Twenty-four hours after LPS injection, the aqueous humor of the right eyes was collected for leukocyte count, protein concentration, and IL-6 assay. Left eyes were processed for routine histology.

RESULTS

TGF beta-1-treated mice showed less ocular inflammation histologically than to the animals that were given PBS. This was confirmed by decreases in leukocyte count, protein concentration, and IL-6 level in the aqueous humor.

CONCLUSIONS

TGF beta-1 inhibits the development of EIU. TGF beta-1 may be useful for the modulation of uveitis in humans.

摘要

目的

转化生长因子β-1（TGFβ-1）可调节炎症。内毒素诱导的葡萄膜炎（EIU）的特征是与包括白细胞介素（IL）-6在内的细胞因子释放相关的急性眼部炎症。作者研究了TGFβ-1对小鼠EIU的影响。

方法

进行了三项独立实验。通过注射200微克脂多糖（LPS）在C3H/HeN小鼠中诱导内毒素诱导的葡萄膜炎。在LPS注射后8小时，将2微克TGFβ-1溶于0.1毫升磷酸盐缓冲盐水（PBS）或仅0.1毫升PBS腹腔注射。LPS注射后24小时，收集右眼房水进行白细胞计数、蛋白质浓度和IL-6检测。左眼进行常规组织学处理。