St-Laurent G, Lepage N, Carman S, Archambault D
Département des sciences biologiques, Université du Québec a Montréal.
Can J Vet Res. 1997 Jan;61(1):72-6.
The genetic variation in equine arteritis virus (EAV) GL protein encoding gene was investigated. Nucleic and deduced amino acid sequences from 7 different EAV isolates, including 4 eastern Canadian field isolates, were compared with those of the Bucyrus reference strain. Nucleotide sequence identities between these isolates and the Bucyrus reference strain ranged from 87.5% (Vienna isolate) to 93.9% (11958 isolate). Amino acid identities with the Bucyrus reference strain varied from 90.2% (Vienna isolate) to 95.1% (19933 isolate). A 2nd potential N-linked glycosylation site was found at position 81 in the GL protein of all EAV isolates. Three amino acid substitutions at residue position 90 (Glu-->Val), position 101 (Ala-->Val or Thr), and position 119 (Val-->Leu, Phe or Ser) were also found in all EAV isolates. Phylogenetic analysis showed that the North American EAV isolates, including the Canadian isolates, and the European prototype Vienna isolate could be classified in 2 distinct groups. Three putative sequential antigenic sites were predicted in EAV GL protein. The 1st antigenic site (TAQRFT) was located at positions 24 to 29, and the 2nd antigenic site (RYDEHTA) at positions 47 to 53. The 3rd antigenic site was predicted to be located at positions 78 to 84 and showed the less conserved amino acid sequence.
对马动脉炎病毒（EAV）GL蛋白编码基因的遗传变异进行了研究。将包括4株加拿大东部野外分离株在内的7株不同EAV分离株的核酸序列和推导的氨基酸序列与Bucyrus参考株进行了比较。这些分离株与Bucyrus参考株之间的核苷酸序列同一性范围为87.5%（维也纳分离株）至93.9%（11958分离株）。与Bucyrus参考株的氨基酸同一性从90.2%（维也纳分离株）到95.1%（19933分离株）不等。在所有EAV分离株的GL蛋白第81位发现了第二个潜在的N-连接糖基化位点。在所有EAV分离株中还发现了第90位（Glu→Val）、第101位（Ala→Val或Thr）和第119位（Val→Leu、Phe或Ser）的三个氨基酸替换。系统发育分析表明，包括加拿大分离株在内的北美EAV分离株和欧洲原型维也纳分离株可分为两个不同的组。在EAV GL蛋白中预测了三个假定的连续抗原位点。第一个抗原位点（TAQRFT）位于第24至29位，第二个抗原位点（RYDEHTA）位于第47至53位。预测第三个抗原位点位于第78至84位，且氨基酸序列保守性较低。
