Feijoó R M, Ardiles A, Lemp P, King A, Villamán J J, Parra A, Silva M
Departamento de Medicina Experimental, Facultad de Medicina, Universidad de Chile (Campus Occidente), Santiago de Chile.
Rev Med Chil. 1996 Mar;124(3):327-36.
The aim of this work was to assess cellular immunity using the Multitest CMI and relate its results with lymphocyte counts and lymphocyte subpopulations determined using monoclonal antibodies against CD4 and CD8 and fluorescence microscopy. We studied 51 patients (31 male), 20 infected with HIV, 18 recurring infections, 5 with cancer, 2 with tuberculosis and 6 with miscellaneous diagnoses. According to Multitest results, patients were classified as normal, hypoergic or anergic. Twenty five percent of patients were normal, 65% hypoergic and 10% anergic. Eighty percent of anergic patients were infected with HIV. No differences in total lymphocyte count were observed between the three groups. CD4 lymphocyte count was lower in anergic patients when compared with the other two groups. All patients with CD4 counts below 200 cells/mm3 were anergic. It is concluded that Multitest CMI is useful for the assessment of cellular immunity and complements the determination of lymphocyte subpopulations.
本研究的目的是使用多重检测CMI评估细胞免疫，并将其结果与使用抗CD4和CD8单克隆抗体及荧光显微镜测定的淋巴细胞计数和淋巴细胞亚群相关联。我们研究了51例患者（31例男性），其中20例感染HIV，18例反复感染，5例患有癌症，2例患有结核病，6例诊断各异。根据多重检测结果，患者被分为正常、低反应性或无反应性。25%的患者为正常，65%为低反应性，10%为无反应性。80%的无反应性患者感染了HIV。三组之间的总淋巴细胞计数未观察到差异。与其他两组相比，无反应性患者的CD4淋巴细胞计数较低。所有CD4计数低于200个细胞/mm³的患者均为无反应性。结论是多重检测CMI有助于评估细胞免疫，并补充淋巴细胞亚群的测定。
