Trebst A, Depka B
Biochemie der Pflanzen, Ruhr Universität, Bochum, Germany.
FEBS Lett. 1997 Jan 6;400(3):359-62. doi: 10.1016/s0014-5793(96)01419-6.
Inhibitors of the phytoene desaturase in carotene biosynthesis were tested in the enhanced rapid turnover of the D1 protein of photosystem II in high light exposure of Chlamydomonas reinhardtii cells. After 1 h high light on heterotrophically grown cells in the presence of norflurazon or fluridone, photosynthesis activity in vivo and PS II activity in vitro is lost. The D1 protein has disappeared. PS I activity is not affected, nor is the D2 protein. It is concluded that beta-carotene is essential for the assembly of the D1 protein into functional photosystem II. It is suspected that bleaching of beta-carotene in the reaction center of PS II by high light destabilizes the structure and triggers the degradation of the D1 protein.
在莱茵衣藻细胞高光暴露条件下，测试了参与胡萝卜素生物合成的八氢番茄红素去饱和酶抑制剂对光系统II中D1蛋白快速周转增强的影响。在异养生长的细胞中，于诺氟唑或氟啶酮存在的情况下进行1小时高光照射后，体内光合作用活性和体外PS II活性丧失。D1蛋白消失。PS I活性未受影响，D2蛋白也未受影响。得出的结论是，β-胡萝卜素对于D1蛋白组装到功能性光系统II中至关重要。据推测，高光使PS II反应中心的β-胡萝卜素漂白，从而破坏结构并引发D1蛋白的降解。
