Rockett J C, Larkin K, Darnton S J, Morris A G, Matthews H R
Department of Biological Sciences, University of Warwick, Coventry, UK.
Br J Cancer. 1997;75(2):258-63. doi: 10.1038/bjc.1997.42.
The derivation of permanent cell lines from 40 resected oesophageal carcinomas has been attempted. Five long-term lines have been established from three adenocarcinomas, one mixed carcinoma and one squamous carcinoma. Molecular and cellular analyses have been carried out on the lines and clones derived from them. Karyotype analysis indicates genetic variation among the clones. HLA-A, -B and -C is expressed constitutively, but not HLA-DR. ICAM-1-expressing phenotypes may have arisen during adaptation to long-term culture. All lines are capable of response to interferon-gamma (IFN-gamma) and all produce transforming growth factor beta 1 (TGF-beta 1). Two lines are resistant to the inhibitory growth effects of the latter, possibly contributing to malignancy. It is anticipated that these lines, originating from histologically different carcinomas, will provide a valuable, continuous resource for the investigation and treatment of these aggressive tumours.
已尝试从40例切除的食管癌中获取永久细胞系。已从3例腺癌、1例混合癌和1例鳞癌中建立了5个长期细胞系。对这些细胞系及其衍生的克隆进行了分子和细胞分析。核型分析表明克隆之间存在遗传变异。HLA - A、- B和- C组成性表达，但HLA - DR不表达。表达细胞间黏附分子- 1（ICAM - 1）的表型可能在适应长期培养过程中出现。所有细胞系都能对γ干扰素（IFN - γ）产生反应，并且都能产生转化生长因子β1（TGF - β1）。有两个细胞系对后者的生长抑制作用具有抗性，这可能促使肿瘤发生恶变。预计这些源自组织学不同癌症的细胞系将为研究和治疗这些侵袭性肿瘤提供宝贵的、持续的资源。
