Shi B J, Ding S W, Symons R H
Department of Plant Science, Waite Institute, University of Adelaide, Australia.
J Gen Virol. 1997 Jan;78 ( Pt 1):237-41. doi: 10.1099/0022-1317-78-1-237.
We recently reported the molecular characterization and functional analysis of an overlapping gene 2b encoded by RNA 2 of the Q strain of cucumber mosaic cucumovirus (Q-CMV). We show here that the homologous gene encoded by the V strain of tomato aspermy cucumovirus (V-TAV) and the WAII strain of CMV (WAII-CMV), which is in a different subgroup to Q-CMV, is also expressed in vivo by demonstrating the accumulation of the mRNA (RNA 4A) and its protein in infected plants. Interestingly, RNA 4A of V-TAV is encapsidated in virions as found previously for Q-CMV whereas WAII-CMV contains very little RNA 4A in virions. As the 2b gene is conserved in all 10 cucumoviral species or strains sequenced to date and the 2b gene is expressed for three of these viruses, we conclude that the 2b gene is a common feature of the Cucumovirus genus.
我们最近报道了黄瓜花叶黄瓜病毒Q株系（Q-CMV）RNA 2编码的重叠基因2b的分子特征及功能分析。我们在此表明，番茄不孕黄瓜病毒V株系（V-TAV）和CMV的WAII株系（WAII-CMV，与Q-CMV属于不同亚组）编码的同源基因，通过证明mRNA（RNA 4A）及其蛋白质在受感染植物中的积累，也在体内表达。有趣的是，V-TAV的RNA 4A如先前在Q-CMV中发现的那样被包装在病毒粒子中，而WAII-CMV的病毒粒子中只含有很少的RNA 4A。由于2b基因在迄今测序的所有10种黄瓜病毒物种或株系中都是保守的，并且其中三种病毒都表达2b基因，我们得出结论，2b基因是黄瓜病毒属的一个共同特征。
