Arizono N, Yamada M, Tegoshi T, Okada M, Uchikawa R, Matsuda S
Department of Medical Zoology, Kyoto Prefectural University of Medicine, Japan.
APMIS. 1994 Aug;102(8):589-96.
Infections of intestinal nematodes induce the T cell-dependent proliferation of intestinal mucosal mast cells (MMC). To examine whether nematode-induced MMC proliferation is affected by the site of infestation, adult-stage nematode Nippostrongylus brasiliensis (NB) was transplanted into the normal infection site, the duodenum, or into heterotopic sites, the peritoneal cavity (i.p.) or subcutaneous tissue (s.c.), of rats. Two weeks after duodenal inoculation, MMC numbers in the small intestine had increased 6.5-fold. In contrast, i.p. and s.c. inoculation induced only slight increases of intestinal MMC. After i.p. inoculation, worm granulomas developed in the connective tissues adhering to stomach and duodenum, and large numbers of mast cells appeared around the granulomas. The majority of the latter mast cells showed histochemical features similar to MMC: they were formalin sensitive, berberine sulfate-, alcian blue+/safranine-, and rat mast cell protease (RMCP) II+. After s.c. inoculation, worm granulomas developed at the inoculation site, but the number of mast cells around the granulomas was not significantly increased. These results suggest that intense proliferation of MMC or MMC-like cells is induced only by the infections on mucosa or in mucosa-associated tissues.
肠道线虫感染会诱导肠道黏膜肥大细胞（MMC）依赖T细胞的增殖。为了研究线虫诱导的MMC增殖是否受感染部位的影响，将成年期巴西日圆线虫（NB）移植到大鼠的正常感染部位十二指肠，或异位部位腹腔（i.p.）或皮下组织（s.c.）。十二指肠接种两周后，小肠中的MMC数量增加了6.5倍。相比之下，腹腔和皮下接种仅引起肠道MMC略有增加。腹腔接种后，在附着于胃和十二指肠的结缔组织中形成蠕虫肉芽肿，并且在肉芽肿周围出现大量肥大细胞。后者的大多数肥大细胞显示出与MMC相似的组织化学特征：它们对福尔马林敏感，对硫酸小檗碱、阿尔辛蓝+/番红、大鼠肥大细胞蛋白酶（RMCP）II呈阳性。皮下接种后，在接种部位形成蠕虫肉芽肿，但肉芽肿周围的肥大细胞数量没有明显增加。这些结果表明，只有黏膜或黏膜相关组织中的感染才会诱导MMC或MMC样细胞的强烈增殖。
