Morin D, Sapena R, Zini R, Onteniente B, Tillement J P
Départment de Pharmacologie, Faculté de Médecine de Paris XII, Créteil, France.
Life Sci. 1997;60(4-5):315-24. doi: 10.1016/s0024-3205(96)00632-7.
The binding and characteristics of rat brain beta-adrenergic receptors (beta-AR) isolated from astrocytes and neurons were investigated. Equilibrium binding experiments demonstrated that beta-AR were more concentrated on astrocytes than on neurons isolated from forebrain, cerebral cortex and cerebellum. Inhibition experiments revealed that beta 1-AR and beta 2-AR were present in the two cell types. Isoproterenol revealed two interchangeable states of high and low affinity binding to both beta 1- and beta 2-AR in neurons. The high affinity binding sites were sensitive to guanylylimidodiphosphate (GppNHp). Similar results were found with other beta-AR agonists but not with salbutamol and salmeterol which recognized both affinity states of the neuronal beta 2-AR but only the low affinity state of beta 1-AR. In astrocytes only the low affinity state of beta-AR was observed.
对从星形胶质细胞和神经元中分离出的大鼠脑β-肾上腺素能受体（β-AR）的结合情况和特性进行了研究。平衡结合实验表明，β-AR在前脑、大脑皮层和小脑中分离出的星形胶质细胞上比在神经元上更集中。抑制实验显示，两种细胞类型中均存在β1-AR和β2-AR。异丙肾上腺素显示神经元中β1-AR和β2-AR均存在高亲和力和低亲和力结合的两种可互换状态。高亲和力结合位点对鸟苷酰亚胺二磷酸（GppNHp）敏感。其他β-AR激动剂也得到了类似结果，但沙丁胺醇和沙美特罗除外，它们识别神经元β2-AR的两种亲和力状态，但只识别β1-AR的低亲和力状态。在星形胶质细胞中，仅观察到β-AR的低亲和力状态。
