Quílez J, Sánchez-Acedo C, Clavel A, del Cacho E, López-Bernad F
Department of Animal Pathology, Parasitology and Parasitic Diseases, Faculty of Veterinary Sciences, University Autónoma of Barcelona, Bellaterra, Spain.
Vet Parasitol. 1996 Dec 2;67(1-2):75-81. doi: 10.1016/s0304-4017(96)01023-0.
A commercially available direct immunofluorescence (IF) assay with monoclonal antibodies (Monofluo Kit Cryptosporidium, Diagnostics Pasteur, France) was compared with the modified Ziehl-Neelsen (MZN) acid-fast technique for the detection of Cryptosporidium oocysts in faecal samples from cattle and pigs. Stool specimens individually collected from 108 bovines and 90 pigs were examined in a blind test. The results of the two procedures corresponded (both positive or negative) in 102 (94.4%) cattle samples and 80 (88.9%) pig faecal samples. However, the remaining six (5.5%) cattle specimens and 10 (11.1%) pig stool samples, all of them harboring few oocysts (0-1 oocysts per 20 x field), were negative by MZN and positive by IF. False-negative results of the acid-fast stain occurred in suckling (17.2% of discrepant results) and weaned calves (2.9%) as well as weaned piglets (43.7%) and fattening pigs (10%). Stool specimens from the remaining age groups were negative by both techniques. The MacNemar's chi-square test showed that differences between both methods were statistically significant (P < 0.05). Compared with immunofluorescence procedure, the sensitivity of MZN technique in samples from cattle and pigs was 79.3% and 67.7% and the negative predictive value was 92.9% and 85.5% respectively. The specificity and positive predictive values of the acid-fast stain were 100% in both animal species. It is concluded that the monoclonal antibody-based immunofluorescence reagent evaluated is more efficient that the MZN technique, especially for detecting a low number of Cryptosporidium oocysts, in faecal specimens from both cattle and pigs.
将一种市售的直接免疫荧光（IF）检测方法（使用单克隆抗体，法国巴斯德诊断公司的Monofluo Kit Cryptosporidium）与改良的齐-尼（MZN）抗酸染色技术进行比较，以检测牛和猪粪便样本中的隐孢子虫卵囊。在一项盲法试验中，对分别从108头牛和90头猪收集的粪便标本进行了检测。两种检测方法的结果在102份（94.4%）牛样本和80份（88.9%）猪粪便样本中一致（均为阳性或均为阴性）。然而，其余6份（5.5%）牛标本和10份（11.1%）猪粪便样本，所有这些样本中隐孢子虫卵囊数量很少（每20×视野0 - 1个卵囊），MZN检测为阴性，而IF检测为阳性。抗酸染色的假阴性结果出现在哺乳犊牛（占差异结果的17.2%）、断奶犊牛（2.9%）以及断奶仔猪（43.7%）和育肥猪（10%）中。其余年龄组的粪便标本两种检测方法均为阴性。麦克尼马尔卡方检验表明，两种方法之间的差异具有统计学意义（P < 0.05）。与免疫荧光检测方法相比，MZN技术在牛和猪样本中的灵敏度分别为79.3%和67.7%，阴性预测值分别为92.9%和85.5%。抗酸染色在两种动物中的特异性和阳性预测值均为100%。结论是，所评估的基于单克隆抗体的免疫荧光试剂比MZN技术更有效，特别是在检测牛和猪粪便标本中数量较少的隐孢子虫卵囊方面。
