Marca M C, Ramos J J, Loste A, Sáez T, Sanz M C
Department of Animal Pathology, Veterinary Faculty, Zaragoza, Spain.
Vet Parasitol. 1996 Dec 2;67(1-2):99-103. doi: 10.1016/s0304-4017(96)01031-x.
A total of 2306 serum samples obtained from adult sheep were analysed for Toxoplasma gondii IgG antibodies by modified direct agglutination test and indirect immunofluorescence assay test. Similar results were obtained with both procedures (seroprevalence of 35.27% for modified direct agglutination test and 33.72% for indirect immunofluorescence assay test at dilution 1:80), but some differences in relation to sample dilution were registered. All the titers assayed (dilution two-fold from 1:40 to 1:320) can be used in diagnosis with both procedures, but titers 1:80 are recommended for screening studies in ovine flocks.
采用改良直接凝集试验和间接免疫荧光试验，对从成年绵羊采集的2306份血清样本进行了弓形虫IgG抗体分析。两种检测方法得到了相似的结果（改良直接凝集试验的血清阳性率为35.27%，间接免疫荧光试验在1:80稀释度下的血清阳性率为33.72%），但在样本稀释方面存在一些差异。两种检测方法均可使用所有测定的滴度（从1:40到1:320两倍稀释）进行诊断，但建议在绵羊群体筛查研究中使用1:80的滴度。
