用于检测家猫体内抗体的间接凝集试验与改良凝集试验的比较。
Comparison of indirect and modified agglutination tests for detection of antibodies to in domestic cats.
作者信息
Fernandes Susana, Brilhante-Simões Paula, Coutinho Teresa, Cardoso Luís, Dubey Jitender P, Lopes Ana P
机构信息
Department of Veterinary Sciences, Laboratory of Parasitology, School of Agrarian and Veterinary Sciences (Fernandes, Coutinho, Cardoso, Lopes), University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal.
Animal and Veterinary Research Centre (CECAV; Cardoso, Lopes), University of Trás-os-Montes e Alto Douro (UTAD), Vila Real, Portugal.
出版信息
J Vet Diagn Invest. 2019 Sep;31(5):774-777. doi: 10.1177/1040638719868753. Epub 2019 Aug 5.
is a protozoan parasite with worldwide distribution. The accurate detection of this zoonotic agent in cats and other hosts has public health importance. Blood samples from 89 domestic cats were tested for antibodies to using 2 commercial agglutination test kits, an indirect (IHAT; Toxo-HAI FUMOUZE; Fumouze Diagnostics) and a modified (MAT; Toxoscreen DA; bioMérieux) agglutination test. Antibodies were found in 16 of 89 (18%) cats by the IHAT and in 23 of 89 (26%) cats by the MAT, with an overall agreement between the 2 serologic tests of 92% (κ = 0.77; i.e., substantial agreement beyond chance). Considering the MAT as the gold standard, the IHAT showed perfect relative specificity (100%) and lower relative sensitivity (70%). The suboptimal sensitivity of the IHAT limits its use in epidemiologic studies in cats.
是一种分布于全球的原生动物寄生虫。在猫和其他宿主中准确检测这种人畜共患病原体具有公共卫生意义。使用两种商业凝集试验试剂盒，即间接血凝试验（IHAT；Toxo-HAI FUMOUZE；Fumouze Diagnostics）和改良凝集试验（MAT；Toxoscreen DA；bioMérieux），对89只家猫的血液样本进行了针对[病原体名称未给出]的抗体检测。通过IHAT在89只猫中的16只（18%）检测到抗体，通过MAT在89只猫中的23只（26%）检测到抗体，两种血清学检测之间的总体一致性为92%（κ = 0.77；即，超出偶然的实质性一致性）。将MAT视为金标准，IHAT显示出完美的相对特异性（100%）和较低的相对敏感性（70%）。IHAT的次优敏感性限制了其在猫流行病学研究中的应用。
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