Callac P, Desmerger C, Kerrigan R W, Imbernon M
Institut National de la Recherche Agronomique, Station de Recherches sur les Champignons, Villenave d'Ornon, France.
FEMS Microbiol Lett. 1997 Jan 15;146(2):235-40. doi: 10.1111/j.1574-6968.1997.tb10199.x.
A previous map of the genome of a hybrid strain which had European parents belonging to the secondarily homothallic fungus Agaricus bisporus var. bisporus appeared to be unusually compact, with a particularly recombophobic segment in the central part of chromosome I. A new map of this segment was constructed based on allelic segregations among 103 homokaryotic offspring of an A. bisporus hybrid between a European parent of the var. bisporus and a Californian parent of the heterothallic var. burnettii. Markers completely linked on the previous map were distributed along 28 cM in the new map. These results suggest that the greater recombination rate could be correlated with the outbreeding behaviour of the var. burnettii.
先前绘制的一种杂交菌株的基因组图谱显示，该菌株的欧洲亲本属于次生同宗配合的真菌双孢蘑菇双孢变种，其基因组图谱异常紧凑，在染色体I的中部有一个特别抗重组的区段。基于双孢变种的欧洲亲本与异宗配合的伯内蒂变种的加利福尼亚亲本之间的双孢蘑菇杂交种的103个同核体后代中的等位基因分离情况，构建了该区段的新图谱。先前图谱上完全连锁的标记在新图谱上分布在28厘摩的范围内。这些结果表明，更高的重组率可能与伯内蒂变种的远交行为相关。
