Larraya L M, Pérez G, Ritter E, Pisabarro A G, Ramírez L
Departamento de Producción Agraria, Universidad Pública de Navarra, Pamplona, Spain.
Appl Environ Microbiol. 2000 Dec;66(12):5290-300. doi: 10.1128/AEM.66.12.5290-5300.2000.
We have constructed a genetic linkage map of the edible basidiomycete Pleurotus ostreatus (var. Florida). The map is based on the segregation of 178 random amplified polymorphic DNA and 23 restriction fragment length polymorphism markers; four hydrophobin, two laccase, and two manganese peroxidase genes; both mating type loci; one isozyme locus (est1); the rRNA gene sequence; and a repetitive DNA sequence in a population of 80 sibling monokaryons. The map identifies 11 linkage groups corresponding to the chromosomes of P. ostreatus, and it has a total length of 1,000.7 centimorgans (cM) with an average of 35.1 kbp/cM. The map shows a high correlation (0.76) between physical and genetic chromosome sizes. The number of crossovers observed per chromosome per individual cell is 0.89. This map covers nearly the whole genome of P. ostreatus.
我们构建了食用担子菌平菇（佛罗里达变种）的遗传连锁图谱。该图谱基于178个随机扩增多态性DNA和23个限制性片段长度多态性标记的分离情况；四个疏水蛋白、两个漆酶和两个锰过氧化物酶基因；两个交配型位点；一个同工酶位点（est1）；rRNA基因序列；以及80个同胞单核体群体中的一个重复DNA序列。该图谱识别出11个与平菇染色体相对应的连锁群，全长为1000.7厘摩（cM），平均每厘摩为35.1千碱基对（kbp）。该图谱显示物理染色体大小与遗传染色体大小之间具有高度相关性（0.76）。每个细胞每个染色体观察到的交叉数为0.89。该图谱几乎覆盖了平菇的整个基因组。
