Zedda M, Farina V
Institute of Veterinary Anatomy, University of Sassari, Italy.
Anat Histol Embryol. 1996 Dec;25(4):257-62. doi: 10.1111/j.1439-0264.1996.tb00089.x.
Cytokeratins (CKs) are a multigenic family of proteins constituting intermediate filaments in epithelia, indicated in humans by the numbers 1-20. Different cell-types can be immunocytochemically identified on the grounds of their CK expression. This investigation was designed to study CK expression of basket cells (BCs) and basal-duct cells (BDCs) in some domestic animals. Frozen sections of mammary and major salivary glands from cows, sheep, pigs and rabbits were treated using the immunofluorescent method, using as monoclonal antibodies clones CK-E3, CKB1, KS-1A3, and LDS-68, respectively, revealing the human CKs 17, 14, 13, 7. BCs surrounding acini and BDCs were stained by CK 17 antibody only in the rabbit. CK 14 was detectable in both cell types in cows, sheep and pigs, except in the case of bovine salivary BCs. CK 13 was revealed in BCs and BDCs of all mammary glands and also rabbit salivary glands. In the salivary glands of the other species, only BDCs were stained. CK 7 gave unreliable results in all the species and cell types examined. Interestingly, in the rabbit, also BDCs are basket-like in shape. The antibodies employed showed different staining depending on species and gland. On the grounds of immunoreactivity and shape, BCs and BDCs can be considered the same cell type in the rabbit. In the other species, they appear to be different, since BDCs may express additional CKs and are triangular-shaped, whereas BCs are truly basket-like. It is worth noting that clone KS-1A3 in the rabbit and CKB1 in the sheep and pig can be considered markers of the basket/ basal system.
细胞角蛋白（CKs）是一个多基因家族的蛋白质，构成上皮细胞中的中间丝，在人类中用数字1 - 20表示。不同的细胞类型可以根据其CK表达通过免疫细胞化学方法进行鉴定。本研究旨在研究一些家畜中篮状细胞（BCs）和基底导管细胞（BDCs）的CK表达。使用免疫荧光法对来自牛、羊、猪和兔的乳腺和主要唾液腺的冰冻切片进行处理，分别使用单克隆抗体克隆CK - E3、CKB1、KS - 1A3和LDS - 68，它们分别揭示人细胞角蛋白17、14、13、7。仅在兔中，腺泡周围的BCs和BDCs被CK 17抗体染色。在牛、羊和猪的两种细胞类型中均可检测到CK 14，但牛唾液腺的BCs除外。在所有乳腺以及兔唾液腺的BCs和BDCs中均检测到CK 13。在其他物种的唾液腺中，仅BDCs被染色。在所有检测的物种和细胞类型中，CK 7给出的结果不可靠。有趣的是，在兔中，BDCs也是篮状的。所使用的抗体根据物种和腺体显示出不同的染色情况。基于免疫反应性和形状，在兔中BCs和BDCs可被视为同一细胞类型。在其他物种中，它们似乎不同，因为BDCs可能表达额外的CKs且呈三角形，而BCs是真正的篮状。值得注意的是，兔中的克隆KS - 1A3以及羊和猪中的CKB1可被视为篮状/基底系统的标志物。
