Draeger A, Nathrath W B, Lane E B, Sundström B E, Stigbrand T I
Pathologisches Institut, Universität, München F.R. Germany.
APMIS. 1991 May;99(5):405-15. doi: 10.1111/j.1699-0463.1991.tb05169.x.
The distribution of immunostaining in normal major salivary gland and in 12 pleomorphic adenomas was studied using monospecific monoclonal antibodies to a number of cytokeratins, including cytokeratin 14, to smooth muscle actin and vimentin. A number of these antibodies enabled a distinction to be made between structural components of the normal gland, and to relate this to the different structures of pleomorphic adenomas. In the normal gland, the luminal duct cells expressed cytokeratins 7, 8, 18 and 19. Three antibodies were of particular value for the characterization of normal myoepithelial and basal cells; while the antibody to smooth muscle actin and the cytokeratin antibody Ks8.12 mutually exclusively stained the myoepithelial (basket) cells and the basal duct (light) cells, respectively, the recently established monospecific antibodies to cytokeratin 14 showed specific immunostaining with both cell types. These three antibodies left luminal cells virtually unstained. Ck 13 was found occasionally in single luminal excretory duct cells. Antibodies to cytokeratins 1/2, 10 and 10/11 did not show any staining in the normal gland. In the pleomorphic adenomas, the staining pattern of the two-layered tubular formation resembled that of the normal gland ducts: tumour luminal cells showed the characteristic, although more irregular, expression of cytokeratins 7, 8, 18 and 19; the outer cells resembled normal ductal basal cells with their anti-cytokeratin 14/Ks8.12-epitope staining and in that they virtually lacked staining for smooth muscle actin. Trabecular formations and cells in myxoid areas were reactive with Ks8.12 and for cytokeratin 14, occasionally also for cytokeratins 7, 18 and 19. Epidermoid cell islets expressed mainly cytokeratin 14 and inconsistently the squamous epithelial cytokeratin 13 and the epidermal cytokeratin 10/11. Vimentin was found in cells of myxoid areas. The results support the postulate that some of the normal duct basal cells act as reserve cells and can give rise to tumour formation with a primitive myxoid or trabecular pattern and a more differentiated tubular or epidermoid configuration.
利用针对多种细胞角蛋白（包括细胞角蛋白14）、平滑肌肌动蛋白和波形蛋白的单特异性单克隆抗体，研究了正常大唾液腺和12例多形性腺瘤中免疫染色的分布情况。其中一些抗体能够区分正常腺体的结构成分，并将其与多形性腺瘤的不同结构联系起来。在正常腺体中，管腔导管细胞表达细胞角蛋白7、8、18和19。三种抗体对正常肌上皮细胞和基底细胞的特征性鉴定具有特殊价值；平滑肌肌动蛋白抗体和细胞角蛋白抗体Ks8.12分别对肌上皮（篮状）细胞和基底导管（亮）细胞进行特异性染色，而最近建立的细胞角蛋白14单特异性抗体对这两种细胞类型均显示特异性免疫染色。这三种抗体几乎未使管腔细胞染色。细胞角蛋白13偶尔见于单个管腔排泄导管细胞中。细胞角蛋白1/2、10和10/11的抗体在正常腺体中未显示任何染色。在多形性腺瘤中，双层管状结构的染色模式类似于正常腺管：肿瘤管腔细胞显示细胞角蛋白7、8、18和19的特征性表达，尽管更不规则；外层细胞与正常导管基底细胞相似，具有抗细胞角蛋白14/Ks8.12表位染色，且几乎缺乏平滑肌肌动蛋白染色。小梁结构和黏液样区域的细胞对Ks8.12和细胞角蛋白14有反应，偶尔对细胞角蛋白7、18和19也有反应。表皮样细胞岛主要表达细胞角蛋白14，偶尔表达鳞状上皮细胞角蛋白13和表皮细胞角蛋白10/11。波形蛋白见于黏液样区域的细胞中。结果支持这样的假设，即一些正常导管基底细胞作为储备细胞，可形成具有原始黏液样或小梁模式以及更分化的管状或表皮样结构的肿瘤。
