Wrenzycki C, Carnwath J W
Institut für Tierzucht und Tierverhalten (FAL), Mariensee.
Dtsch Tierarztl Wochenschr. 1996 Aug-Sep;103(8-9):316-20.
Molecular biological methods developed for basic research have been increasingly employed in animal production. For example, individual animals can be identified by their unique DNA sequences in DNA fingerprints and their genetic relationships can be established by more detailed sequence comparisons. It is also possible to diagnose gene defects and to determine the sex of preimplantation embryos. This information can then be used as the basis for an efficient breeding program. Methods for the analysis of RNA yield information about genetic or environmental factors which influence the expression of specific genes for example genes of economic value in milk production or genes which are important for preimplantation embryo development. This information will enable further improvements in embryo transfer and associated biotechniques which promise to improve the rate of genetic improvement (NIEMANN and MEINECKE, 1993). In the following section, we will briefly describe these molecular biological methods and their applications in animal breeding using examples from current research in our research group.
为基础研究而开发的分子生物学方法已越来越多地应用于动物生产。例如,个体动物可通过其DNA指纹中的独特DNA序列进行识别,其遗传关系可通过更详细的序列比较来确定。还能够诊断基因缺陷并确定植入前胚胎的性别。然后,这些信息可作为高效育种计划的基础。分析RNA的方法可提供有关遗传或环境因素的信息,这些因素会影响特定基因的表达,例如牛奶生产中具有经济价值的基因或对植入前胚胎发育很重要的基因。这些信息将有助于进一步改进胚胎移植及相关生物技术,有望提高遗传改良率(尼曼和迈内克,1993年)。在以下部分,我们将通过我们研究小组当前研究的实例,简要描述这些分子生物学方法及其在动物育种中的应用。