Zółtowska K, Kurowicka B, Lukaszewicz D
Katedra Biologii Ogólnej Instytutu Biologii i Ochrony Srodowiska, WSP, Olsztyn.
Wiad Parazytol. 1996;42(3):319-26.
Larvae of Ascaris suum (L2) newly hatched from eggs with sodium hypochlorite, were placed (2000 larvae per 2 ml) of culture in EAGLE's medium, containing 10% calve serum or without it. Moxidectin (Cydectin) was introduced into medium in concentrations: 5, 10, 25 and 50 micrograms/ml. The cultures were incubated for 3 days, and there were controlled every 12 hours. The effect of drug on survival of larvae was slightly expressed. After 3 days survival rates were 87, 81, 80 and 78% in medium with serum, respectively to moxidectin concentrations. The lethality of larvae was higher in medium without serum, and amounted to 17, 23, 29 and 31% in comparison with control probe after 72 hours. Adult Ascaris were placed on ARS medium with glucose (0.1%) containing moxidectin in concentrations 1, 5, 50 and 100 micrograms/ml. The worms' condition was examined every day based on motility and turgor of their body. After 14 days of incubation in control probe 50% worms were alive, but their vitality was reduced. The behaviour of Ascaris in the presence of 50 micrograms/ml moxidectin was similar to those from the control. Only the highest concentration of drug (100 micrograms/ml) was lethal in 100% on the 8th day. The lower moxidectin concentrations (1 and 5 micrograms/ml) were not lethal to adult worms, they had even the positive effect on survival rate and condition of adult Ascaris. The eggs laid by females which were maintained in culture with drug were collected. There were no disturbances in their future development.
用次氯酸钠从虫卵中新孵化出的猪蛔虫幼虫（L2），以每2毫升2000条幼虫的密度接种于含有10%小牛血清或不含小牛血清的伊格尔培养基中。将莫西菌素（赛得菌素）以5、10、25和50微克/毫升的浓度加入培养基中。培养物孵育3天，每12小时进行一次观察。药物对幼虫存活的影响不明显。3天后，含血清培养基中幼虫的存活率分别为87%、81%、80%和78%，对应不同的莫西菌素浓度。不含血清的培养基中幼虫的死亡率更高，72小时后与对照样本相比分别为17%、23%、29%和31%。将成年猪蛔虫置于含有浓度为1、5、50和100微克/毫升莫西菌素的含葡萄糖（0.1%）的ARS培养基上。每天根据虫体的活力和饱满度检查虫体状况。在对照样本中孵育14天后，50%的虫体存活，但活力有所下降。在50微克/毫升莫西菌素存在的情况下，猪蛔虫的行为与对照相似。只有最高浓度的药物（100微克/毫升）在第8天具有100%的致死率。较低浓度的莫西菌素（1和5微克/毫升）对成年虫体无致死作用，甚至对成年猪蛔虫的存活率和状况有积极影响。收集在含药物培养基中饲养的雌虫所产的卵。其后续发育未受干扰。
