Tabira T, Webster H F
J Neurol Sci. 1979 Jul;42(2):215-27. doi: 10.1016/0022-510x(79)90053-4.
Aldehyde fixed Xenopus tadpole and frog optic nerves were stained en bloc with ethanolic phosphotungstic acid (E-PTA). During rapid myelination, intense staining was observed on cytoplasmic faces of paranodal terminal loops and loosely wrapped oligodendroglial membranes found along inner and outer surfaces of compact myelin sheaths. Oligodendroglial microtubules also were heavily stained. Where stained cytoplasmic faces fused to form a lamella of compact myelin, the intense staining was reduced to a thinner, fainter line. In optic nerves of adult frogs, the staining was less dense but the pattern was similar. The staining distribution and available histochemical evidence indicate that E-PTA stains positively charged proteins non specifically. Since myelin basic protein is found in oligodendroglia during myelination, we suggest that it is being stained by E-PTA while being transported along microtubules to sites where it is inserted into developing myelin lamellae.
用乙醇磷钨酸（E-PTA）对醛固定的非洲爪蟾蝌蚪和青蛙的视神经进行整体染色。在快速髓鞘形成过程中，在节旁终末环的细胞质面以及沿致密髓鞘内、外表面发现的松散包裹的少突胶质细胞膜上观察到强烈染色。少突胶质细胞微管也被严重染色。当染色的细胞质面融合形成致密髓鞘板层时，强烈染色减少为更细、更淡的线。在成年青蛙的视神经中，染色密度较低，但模式相似。染色分布和现有的组织化学证据表明，E-PTA非特异性地染色带正电荷的蛋白质。由于在髓鞘形成过程中在少突胶质细胞中发现了髓鞘碱性蛋白，我们认为它在沿微管运输到插入发育中的髓鞘板层的部位时被E-PTA染色。
