Lepper A W, Newton-Tabrett D A, Corner L A, Carpenter M T, Scanlan W A, Williams O J, Helwig D M
Aust Vet J. 1977 May;53(5):208-13. doi: 10.1111/j.1751-0813.1977.tb00187.x.
The efficiency of 2 different doses of bovine PPD tuberculin was compared using the caudal fold test for the detection of tuberculosis in beef cattle. Two matched groups of 98 cattle were selected on the basis of their reactivity to HCSM tuberculin. Cattle in each group were tested with a singl 0.1 ml dose of bovine PPD tuberculin containing either 0.1 mg or 0.2mg bovine PPD respectively. Two further groups of 100 young stock from a herd with an incidence of tuberculosis of less than 0.1% were selected as controls. Tests were interpreted subjectively by palpation and observation and objectively by caliper measurement at 48, 72 and 96h. All cattle were examined post mortem for the presence of visible lesions. The 0.2 mg dose of PPD detected 95% tuberculous cattle and caused false-positive reactions in 19% of cattle without lesions. The 0.1 mg dose detected 80% of tuberculous cattle and caused 11% false-positive reactions. No visible lesions of tuberculosis were found in either of the control groups although 11% reacted to 0.2 mg and 5% to 0.1 mg bovine PPD at 72h. The optimum time for reading tuberculin tests was found to be in the period 48 to 72h after the injection of bovine PPD and subjective interpretation was more efficient than caliper measurement as a means of detection of tuberculous cattle. The 0.2 mg dose of bovine PPD was found to be more specific than HCSM tuberculin.
使用尾褶试验比较了两种不同剂量牛PPD结核菌素在肉牛结核病检测中的效率。根据牛对HCSM结核菌素的反应性，选择了两组各98头匹配的牛。每组牛分别用0.1ml单剂量的牛PPD结核菌素进行检测，其中一种含0.1mg牛PPD，另一种含0.2mg牛PPD。另外从结核病发病率低于0.1%的牛群中选择两组各100头幼畜作为对照。在48、72和96小时通过触诊和观察进行主观解读，并通过卡尺测量进行客观解读。所有牛在死后进行检查，以确定是否存在可见病变。0.2mg剂量的PPD检测出95%的结核病牛，并在19%无病变的牛中引起假阳性反应。0.1mg剂量检测出80%的结核病牛，并引起11%的假阳性反应。两个对照组均未发现明显的结核病变，尽管在72小时时，11%的牛对0.2mg牛PPD有反应，5%的牛对0.1mg牛PPD有反应。发现读取结核菌素试验的最佳时间是在注射牛PPD后的48至72小时内，作为检测结核病牛的一种方法，主观解读比卡尺测量更有效。发现0.2mg剂量的牛PPD比HCSM结核菌素更具特异性。
