Fenton B, Malloch G, Moxey E
Scottish Crop Research Institute, Invergowrie, Dundee, Scotland.
Insect Mol Biol. 1997 Feb;6(1):23-32. doi: 10.1046/j.1365-2583.1997.00152.x.
Ribosomal DNA internal transcribed spacers of the eriophyid mites Cecidophyopsis ribis, C. selachodon, C. spicata, C. alpina, C. aurea, C. grossulariae and Phylocoptes gracillis were amplified using PCR, cloned and sequenced. Sequences for the ITS1 of Cecidophyopsids were 92-99% homologous. Cecidophyopsis inter-specific differences were found in seventeen simple sequence repeats (vSSRs), fourteen point mutations and two indels. No intra-specific variation in vSSRs was detected. A hypothetical structure for ITS1 was obtained and vSSRs were mapped onto this. Changes in vSSRs were compensated for by changes in complementary vSSRs or through multiple point mutations. A comparison with vSSRs of other arthropods suggested that the levels of intra-specific variation in Cecidophyopsis mites was less than in organisms which do not use arrhentoky for male determination.
利用聚合酶链式反应（PCR）扩增、克隆并测序了叶螨科的梨叶刺瘿螨（Cecidophyopsis ribis）、鲨栖刺瘿螨（C. selachodon）、穗状刺瘿螨（C. spicata）、高山刺瘿螨（C. alpina）、金色刺瘿螨（C. aurea）、醋栗刺瘿螨（C. grossulariae）和细弱叶螨（Phylocoptes gracillis）的核糖体DNA内转录间隔区。刺瘿螨属的ITS1序列同源性为92%-99%。在十七个简单序列重复（vSSR）、十四个点突变和两个插入/缺失中发现了刺瘿螨属种间差异。未检测到vSSR的种内变异。获得了ITS1的假设结构，并将vSSR映射到该结构上。vSSR的变化通过互补vSSR的变化或多个点突变得到补偿。与其他节肢动物的vSSR比较表明，刺瘿螨种内变异水平低于不以产雄孤雌生殖来决定性别的生物。
