Lewandowski Mariusz, Skoracka Anna, Szydło Wiktoria, Kozak Marcin, Druciarek Tobiasz, Griffiths Don A
Department of Applied Entomology, Faculty of Horticulture, Biotechnology and Landscape Architecture, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776, Warsaw, Poland,
Exp Appl Acarol. 2014 Aug;63(4):497-520. doi: 10.1007/s10493-014-9805-z. Epub 2014 Apr 8.
Eriophyoid species belonging to the genus Trisetacus are economically important as pests of conifers. A narrow host specialization to conifers and some unique morphological characteristics have made these mites interesting subjects for scientific inquiry. In this study, we assessed morphological and genetic variation of seven Trisetacus species originating from six coniferous hosts in Poland by morphometric analysis and molecular sequencing of the mitochondrial cytochrome oxidase subunit I gene and the nuclear D2 region of 28S rDNA. The results confirmed the monophyly of the genus Trisetacus as well as the monophyly of five of the seven species studied. Both DNA sequences were effective in discriminating between six of the seven species tested. Host-dependent genetic and morphological variation in T. silvestris and T. relocatus, and habitat-dependent genetic and morphological variation in T. juniperinus were detected, suggesting the existence of races or even distinct species within these Trisetacus taxa. This is the first molecular phylogenetic analysis of the Trisetacus species. The findings presented here will stimulate further investigations on the evolutionary relationships of Trisetacus as well as the entire Phytoptidae family.
属于毛刺瘿螨属的瘿螨种类作为针叶树害虫具有重要经济意义。对针叶树的狭窄寄主专一性以及一些独特的形态特征，使这些螨类成为科学研究的有趣对象。在本研究中，我们通过形态测量分析以及线粒体细胞色素氧化酶亚基I基因和28S rDNA核D2区域的分子测序，评估了源自波兰六种针叶树寄主的七种毛刺瘿螨种类的形态和遗传变异。结果证实了毛刺瘿螨属的单系性以及所研究的七种中的五种的单系性。两种DNA序列在区分所测试的七种中的六种时均有效。检测到了欧洲毛刺瘿螨和迁移毛刺瘿螨中寄主依赖的遗传和形态变异，以及杜松毛刺瘿螨中栖息地依赖的遗传和形态变异，这表明在这些毛刺瘿螨分类群中存在族甚至不同的物种。这是对毛刺瘿螨种类的首次分子系统发育分析。此处呈现的研究结果将推动对毛刺瘿螨以及整个瘿螨科进化关系的进一步研究。
