Barbé E, Huitinga I, Döpp E A, Bauer J, Dijkstra C D
Department of Pathology, Academic Hospital of The Vrije Universiteit, The Netherlands.
J Cell Sci. 1996 Dec;109 ( Pt 12):2937-45. doi: 10.1242/jcs.109.12.2937.
Bone marrow macrophages are found in intimate contact with erythroblasts. Exact mechanisms and functions of this interaction are unclear. New insights into erythroblast binding were obtained using a newly developed bone marrow frozen section assay. This modified Woodruff and Stamper assay has some important advantages compared to other adhesion assays. Erythroblasts specifically adhered to bone marrow macrophages forming clusters, as appear in vivo. Selective depletion of bone marrow macrophages by intravenous injection of dichloromethyl-enediphosphonate resulted in a release of immature erythroid cells to the peripheral blood. Furthermore no erythroblasts adhered to bone marrow sections without macrophages. Evaluating the binding of erythroblasts to bone marrow macrophages we found that this binding is temperature and cation dependent. The receptor for erythroblasts present on macrophages recognizes a sialyated protein as ligand on erythroblasts, since neuraminidase treatment of erythroblasts resulted in a decrease in binding. A possible candidate for the erythroblast receptor on macrophages is the ED2 antigen. ED2 is a differentiation antigen present on resident macrophages that has some biochemical features characteristic of an adhesion molecule. Erythroblast binding to bone marrow was inhibited using a monoclonal antibody directed against ED2.
骨髓巨噬细胞与成红细胞紧密接触。这种相互作用的确切机制和功能尚不清楚。使用新开发的骨髓冰冻切片试验获得了关于成红细胞结合的新见解。与其他黏附试验相比，这种改良的伍德拉夫和斯坦珀试验有一些重要优势。成红细胞特异性黏附于骨髓巨噬细胞并形成簇，如同在体内出现的那样。通过静脉注射二氯亚甲基二膦酸盐选择性清除骨髓巨噬细胞，导致未成熟红细胞释放到外周血中。此外，没有巨噬细胞的骨髓切片上没有成红细胞黏附。评估成红细胞与骨髓巨噬细胞的结合时，我们发现这种结合依赖于温度和阳离子。巨噬细胞上存在的成红细胞受体识别成红细胞上的一种唾液酸化蛋白作为配体，因为用神经氨酸酶处理成红细胞会导致结合减少。巨噬细胞上成红细胞受体的一个可能候选者是ED2抗原。ED2是驻留巨噬细胞上存在的一种分化抗原，具有一些黏附分子特有的生化特征。使用针对ED2的单克隆抗体可抑制成红细胞与骨髓的结合。
