Suri A, Kaltenbach M L, Grundy B L, Derendorf H
Department of Pharmaceutics, College of Pharmacy, University of Florida, Gainesville 32610, USA.
J Clin Pharmacol. 1996 Dec;36(12):1126-31. doi: 10.1002/j.1552-4604.1996.tb04166.x.
Pain assessment in human volunteers is difficult, and it often requires a large number of subjects to show analgesic efficacy with statistical significance. Electrical tooth pulp stimulation elicits a painful sensation and produces an electroencephalographic (EEG) signal that can be recorded from the scalp when precisely controlled dental stimuli are delivered. These somatosensory evoked potentials (EP) consist of a series of peaks or waves each characterized by their polarity, latency, and amplitude. They are obtained by processing the EEG signals that occur after tooth pulp stimulation. There is good correlation between subjective pain reports and evoked potential amplitudes (N150-P250 component). Thus, EP may provide a useful model for the assessment of analgesic activity in human volunteers. We describe an improved method for producing and recording tooth pulp evoked potentials in six healthy subjects. Only 16 EEG epochs were necessary to get a reproducible EP response from the participants. The approach was applied to study the efficacy of codeine (60 mg administered orally); a decrease in the evoked potential amplitudes after codeine administration was observed. The data were consistent with results from visual analog pain ratings given by the subjects.
在人类志愿者中进行疼痛评估很困难，通常需要大量受试者才能显示出具有统计学意义的镇痛效果。当精确控制牙齿刺激时，电刺激牙髓会引发疼痛感觉并产生可从头皮记录的脑电图（EEG）信号。这些体感诱发电位（EP）由一系列峰或波组成，每个峰或波都以其极性、潜伏期和振幅为特征。它们是通过处理牙髓刺激后出现的EEG信号获得的。主观疼痛报告与诱发电位振幅（N150-P250成分）之间存在良好的相关性。因此，EP可能为评估人类志愿者的镇痛活性提供一个有用的模型。我们描述了一种在六名健康受试者中产生和记录牙髓诱发电位的改进方法。仅需16个EEG时段就能从参与者那里获得可重复的EP反应。该方法被用于研究可待因（口服60毫克）的疗效；观察到服用可待因后诱发电位振幅降低。这些数据与受试者给出的视觉模拟疼痛评分结果一致。
