Quesada M A
Biology Department, Brookhaven National Laboratory, Upton, NY 11973, USA.
Curr Opin Biotechnol. 1997 Feb;8(1):82-93. doi: 10.1016/s0958-1669(97)80162-0.
Much progress has been made in the development of replaceable sieving polymers and capillary coatings for high-performance DNA sequencing by capillary electrophoresis. Several studies of parameters that affect resolution, read length and reproducibility have begun to reveal the physical mechanisms acting on single-stranded DNA during electrophoresis through semidilute polymer solutions. Recently developed electro-osmosis-inhibiting matrix polymers have simplified the process of coating capillaries, facilitating the automation of high-throughput parallel systems for large-scale sequencing.
在用于毛细管电泳的高性能DNA测序的可替换筛分聚合物和毛细管涂层的开发方面已经取得了很大进展。几项关于影响分辨率、读取长度和重现性的参数的研究已经开始揭示在通过半稀释聚合物溶液进行电泳期间作用于单链DNA的物理机制。最近开发的抑制电渗的基质聚合物简化了毛细管涂层的过程，促进了用于大规模测序的高通量并行系统的自动化。
