Thompson N L, Lagerholm B C
Department of Chemistry, Campus Box 3290, University of North Carolina, Chapel Hill, NC 27599-3290, USA.
Curr Opin Biotechnol. 1997 Feb;8(1):58-64. doi: 10.1016/s0958-1669(97)80158-9.
Molecular interactions occurring on or near cell membrane surfaces are expected to have different properties from those occurring in bulk solutions. One particularly useful technique for studying surface-associated processes at the molecular level is total internal reflection fluorescence. In this method, the evanescent field from an internally reflected excitation source selectively excites fluorescent molecules on or near a surface. Evanescent excitation has been used recently with a variety of techniques in fluorescence microscopy and spectroscopy to probe the fundamental physicochemical properties of biochemical reactions at natural or model biological surfaces. These studies are providing enhanced understanding of cellular function. Several recent developments in total internal reflection fluorescence methodology from other fields are likely to find future application in cellular biophysics.
预计发生在细胞膜表面或其附近的分子相互作用具有与发生在本体溶液中的分子相互作用不同的特性。全内反射荧光是一种在分子水平上研究表面相关过程的特别有用的技术。在这种方法中，来自内反射激发源的倏逝场选择性地激发表面上或附近的荧光分子。最近，倏逝激发已与荧光显微镜和光谱学中的各种技术一起使用，以探测天然或模型生物表面上生化反应的基本物理化学性质。这些研究正在加深对细胞功能的理解。来自其他领域的全内反射荧光方法学的一些最新进展可能会在细胞生物物理学中得到未来应用。
