老年自发性高血压大鼠连合下器官中碱性成纤维细胞生长因子的缺失。

Loss of basic fibroblast growth factor in the subcommissural organ of old spontaneously hypertensive rats.

作者信息

Cuevas P, Reimers D, Giménez-Gallego G

机构信息

Servicio de Histología, Hospital Universitario Ramón y Cajal, Madrid, Spain.

出版信息

Neurosci Lett. 1996 Dec 27;221(1):25-8. doi: 10.1016/s0304-3940(96)13277-8.

DOI:10.1016/s0304-3940(96)13277-8

PMID:9014172

Abstract

The immunocytochemical localization of basic fibroblast growth factor (bFGF) was studied in subcommissural organ (SCO) of aged-matched normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive (SH) rats at 10, 14 and 18 months of age using a polyclonal antibody against bFGF. The bFGF-like immunoreactivity (bFGF-ir) was observed in SCO ependymal cells of young and old normotensive rats. However, a progressive loss of bFGF-immunopositive ependymal SCO cells was observed with age in SH rats (27.24, 57.5 and 96.9% in 10, 14 and 18 months old respectively) compared with aged-matched WKY normotensive rats. Considering the potential role of the SCO in sleep regulation and sodium homeostasis, which are altered in essential hypertension, these data show a new neuroendocrine anomaly to be added to the many others previously observed in this rat strain, when they develop hypertension as they get old.

摘要

使用抗碱性成纤维细胞生长因子（bFGF）的多克隆抗体，研究了10、14和18月龄年龄匹配的正常血压Wistar-Kyoto（WKY）大鼠和自发性高血压（SH）大鼠的室管膜下器官（SCO）中碱性成纤维细胞生长因子（bFGF）的免疫细胞化学定位。在年轻和老年正常血压大鼠的SCO室管膜细胞中观察到bFGF样免疫反应性（bFGF-ir）。然而，与年龄匹配的WKY正常血压大鼠相比，SH大鼠中随着年龄增长，bFGF免疫阳性的SCO室管膜细胞逐渐减少（10、14和18月龄分别为27.24%、57.5%和96.9%）。考虑到SCO在睡眠调节和钠稳态中的潜在作用，而这些在原发性高血压中会发生改变，这些数据表明，当这种大鼠品系随着年龄增长出现高血压时，在先前观察到的许多其他异常之外，又出现了一种新的神经内分泌异常。