Peitsch M C, Herzyk P, Wells T N, Hubbard R E
BioInformatics, Glaxo Institute for Molecular Biology, Geneva, Switzerland.
Recept Channels. 1996;4(3):161-4.
Molecular modelling of the transmembrane helices of G-protein coupled receptors is an increasingly used method to identify the possible three-dimensional environment of key residues. Thereby site-directed mutagenesis experiments, aimed at the understanding of the receptor-ligand interactions, can be designed in a rational way. The modelling methods are however not generally available to experimentalists, and often require expensive software and hardware. To overcome these limitations, we have constructed a World Wide Web server for the automated protein modelling of user-defined transmembrane helices. The service is freely available at this address: http:/(/)expasy.hcuge.ch/swissmod/SWISS-MODEL.++ +html.
G蛋白偶联受体跨膜螺旋的分子建模是一种越来越常用的方法，用于确定关键残基可能的三维环境。由此，可以合理设计旨在理解受体-配体相互作用的定点诱变实验。然而，建模方法并非实验人员普遍可用，并且通常需要昂贵的软件和硬件。为了克服这些限制，我们构建了一个万维网服务器，用于对用户定义的跨膜螺旋进行自动蛋白质建模。该服务可在以下地址免费获取：http:/(/)expasy.hcuge.ch/swissmod/SWISS-MODEL.++ +html。
