Liebler E M, Küsters C, Pohlenz J F
Department of Veterinary Pathology, Veterinary School Hannover, Germany.
Vet Immunol Immunopathol. 1996 Dec;55(1-3):93-105. doi: 10.1016/s0165-2427(96)05715-7.
Changes in the number of lymphocyte and plasma cell subtypes were investigated in the lamina propria and in the epithelium of the small and large intestine of cattle with mucosal disease. Mucosal disease had been induced experimentally in seven out of 13 animals persistently viremic with non cytopathogenic BVD-virus by inoculation with a matching cytopathogenic BVD-virus. For comparison, six clinically healthy, persistently viremic cattle were used. IgA+, IgM+ and IgG1+ plasma cells, BoCD4+, BoCD8+ and gamma delta + T-lymphocytes, and the antigen of the cytopathogenic BVD-virus were demonstrated in tissue sections by immunohistochemistry. Distribution of cellular subtypes in the controls was consistent with data reported from non infected cattle. In cattle with mucosal disease, a decrease in the number of plasma cells which was significant for IgA+ and IgM+, but not for IgG1+ plasma cells was found in the lamina propria. The number of BoCD4+ T-lymphocytes was reduced in the small intestine, whereas their number per mm2 of mucosa was increased in the large intestine. Numbers of intraepithelial BoCD8+ and gamma delta + T-lymphocytes were severely decreased. Antigen of the cytopathogenic BVD-virus was detected predominantly in epithelial cells of the crypts. Overall there is a severe loss of effector cells which are essential components of the humoral and cell mediated immune protection of the mucosal barrier. The decrease of immunoregulatory cells in the lamina propria and epithelium may contribute to the transformation of mucosal architecture in mucosal disease.
对患有黏膜病的牛的小肠和大肠固有层及上皮中淋巴细胞和浆细胞亚型数量的变化进行了研究。通过接种匹配的细胞病变性牛病毒性腹泻病毒（BVD - 病毒），在13只持续病毒血症且携带非细胞病变性BVD - 病毒的动物中，有7只被实验性诱导出黏膜病。作为对照，使用了6头临床健康、持续病毒血症的牛。通过免疫组织化学在组织切片中显示了IgA +、IgM +和IgG1 +浆细胞、BoCD4 +、BoCD8 +和γδ + T淋巴细胞以及细胞病变性BVD - 病毒的抗原。对照组中细胞亚型的分布与未感染牛的报道数据一致。在患有黏膜病的牛中，固有层中浆细胞数量减少，对于IgA +和IgM +浆细胞而言差异显著，但对于IgG1 +浆细胞则不显著。小肠中BoCD4 + T淋巴细胞数量减少，而大肠中每平方毫米黏膜中的数量增加。上皮内BoCD8 +和γδ + T淋巴细胞数量严重减少。细胞病变性BVD - 病毒的抗原主要在隐窝的上皮细胞中检测到。总体而言，作为黏膜屏障体液免疫和细胞介导免疫保护重要组成部分的效应细胞严重缺失。固有层和上皮中免疫调节细胞的减少可能有助于黏膜病中黏膜结构的转变。
