Parsons K R, Hall G A, Bridger J C, Cook R S
AFRC Institute for Animal Health, Compton, Newbury, UK.
Vet Immunol Immunopathol. 1993 Dec;39(4):355-64. doi: 10.1016/0165-2427(93)90067-e.
An understanding of the immune response to rotavirus is needed to develop effective prophylaxis. There is evidence that cell-mediated responses may be involved and to extend these observations, rotavirus antigen and the three major T cell subsets, BoCD4+, BoCD8+, and BoWC1+ gamma/delta lymphocytes were immunostained in tissue sections from calves killed at 2, 4, 6, 8 and 10 days post inoculation and quantified by image analysis. It was established that in control calves, BoCD4+ lymphocytes were predominantly in the lamina propria, while the majority of BoCD8+ and BoWC1+ gamma/delta lymphocytes were in the epithelium. Rotavirus infection was seen throughout the small intestine with the greatest amount of viral antigen detected at 4 days post inoculation in the mid and distal small intestine. Increased numbers of all subsets were detected; small increases in intraepithelial BoCD4+ and BoWC1+ gamma/delta T lymphocytes were observed especially in the distal small intestine, while larger increases in BoCD8+ cells were detected in the epithelium and lamina propria of the proximal, mid and distal small intestine. The timing and location of these increases in T lymphocyte subsets is indicative of a specific immune response involving BoCD8+ and BoWC1+ gamma/delta T lymphocytes.
为了开发有效的预防措施，需要了解对轮状病毒的免疫反应。有证据表明细胞介导的反应可能参与其中，为了扩展这些观察结果，对接种后2、4、6、8和10天处死的小牛的组织切片中的轮状病毒抗原以及三种主要的T细胞亚群（BoCD4 +、BoCD8 +和BoWC1 +γ/δ淋巴细胞）进行了免疫染色，并通过图像分析进行定量。结果表明，在对照小牛中，BoCD4 +淋巴细胞主要位于固有层，而大多数BoCD8 +和BoWC1 +γ/δ淋巴细胞位于上皮细胞中。在整个小肠中都发现了轮状病毒感染，在接种后4天，在小肠中部和远端检测到的病毒抗原量最多。所有亚群的数量均增加；观察到上皮内BoCD4 +和BoWC1 +γ/δT淋巴细胞略有增加，尤其是在小肠远端，而在小肠近端、中部和远端的上皮和固有层中，BoCD8 +细胞的增加幅度更大。这些T淋巴细胞亚群增加的时间和位置表明涉及BoCD8 +和BoWC1 +γ/δT淋巴细胞的特异性免疫反应。
