Isoda H, Akagi K, Aoki Y, Tanaka Y
Department of Radiology, Kansai Medical University, Osaka, Japan.
Indian J Exp Biol. 1996 Sep;34(9):898-900.
Ascorbate radical (Asc.-) produced by the reaction of AscH- (ascorbic acid) with HO or O2.- after irradiation in mice has been measured. It is possible to measure Asc.- easily using ESR and a dialysis method in which Asc.- is collected at room temperature in the interstitial fluid through the dialysis membrane. After irradiation, Asc.- increases in both normal muscle and tumor tissues (SCC-VII) in proportion to the radiation dose. These results suggest that the amount of HO and O2.- produced is reflected in the Asc.- production. This method has been found more useful for the following reasons, (i) no special treatment, such as freezing of the sample, and no administration of noxious agents are necessary to measure Asc.-, (ii) irradiation using a dose of only a few Gy shows an increase in production of Asc.-, and (iii) this method dose not require removal of organs. Using this method, Asc.- can serve as an indicator of the amount of HO and O2.- produced by irradiation in vivo.
已对小鼠照射后抗坏血酸（AscH-）与HO或O2.-反应产生的抗坏血酸自由基（Asc.-）进行了测定。使用电子自旋共振（ESR）和透析方法可以轻松测定Asc.-，在该透析方法中，Asc.-在室温下通过透析膜在间质液中收集。照射后，正常肌肉和肿瘤组织（SCC-VII）中的Asc.-均与辐射剂量成比例增加。这些结果表明，产生的HO和O2.-的量反映在Asc.-的产生中。该方法因以下原因而更有用：（i）测量Asc.-时无需对样品进行冷冻等特殊处理，也无需施用有害试剂；（ii）仅使用几Gy的剂量进行照射就会使Asc.-的产生增加；（iii）该方法无需切除器官。使用这种方法，Asc.-可以作为体内照射产生的HO和O2.-量的指标。
