Williams G R, Lu Q L, Love S B, Talbot I C, Northover J M
ICRF Colorectal Unit, St. Mark's Hospital, Northwick Park, Watford Road, Harrow, UK.
J Pathol. 1996 Dec;180(4):378-82. doi: 10.1002/(SICI)1096-9896(199612)180:4<378::AID-PATH697>3.0.CO;2-H.
Evidence of human papillomavirus (HPV) can be found in up to 85 per cent of anal carcinomas. In the vulva, a discrete subset of HPV-positive carcinomas which show koilocytic morphology and distinct clinical features has recently been identified (warty carcinoma). The morphological and prognostic features of HPV-positive and HPV-negative anal carcinomas were compared in this study of the tumour distribution of HPV DNA. Vulval and anal neoplasia are similar in many ways and we have also looked to see if their similarity extends to 'warty' morphology in relation to HPV status. Thirty-five resection specimens of anal carcinoma were examined with biotin-labelled probes for HPV 6, 11, 16, and 18 DNA, using a non-isotopic in situ hybridization (ISH) technique. No tumour was found to contain HPV 6, 11, or 18. Twenty-four (72 per cent) showed positivity for HPV 16 DNA. Staining was homogeneous and independent of local squamous, basaloid, or ductal differentiation. The majority of tumours showed staining suggestive of episomal, non-productive HPV infection. HPV-positive tumours were more likely to occur in the anal canal than perianally and to show a mixed squamous and basaloid appearance. No difference between the two groups was found in patient age, presence of adjacent dysplasia, ductal differentiation, or prognosis. There was no correlation between condylomatous tumour morphology and HPV 16 DNA positivity; thus, a subset equivalent to vulval warty carcinoma could not be identified.
在高达85%的肛管癌中可发现人乳头瘤病毒（HPV）感染迹象。在外阴，最近已鉴定出一个离散的HPV阳性癌亚组，其具有挖空细胞形态和独特的临床特征（疣状癌）。在这项关于HPV DNA肿瘤分布的研究中，对HPV阳性和HPV阴性肛管癌的形态学和预后特征进行了比较。外阴和肛管肿瘤在许多方面相似，我们还研究了它们的相似性是否延伸至与HPV状态相关的“疣状”形态。使用非同位素原位杂交（ISH）技术，用生物素标记的探针检测了35例肛管癌切除标本中的HPV 6、11、16和18 DNA。未发现肿瘤含有HPV 6、11或18。24例（72%）显示HPV 16 DNA阳性。染色均匀，与局部鳞状、基底样或导管分化无关。大多数肿瘤显示的染色提示游离型、非增殖性HPV感染。HPV阳性肿瘤更可能发生在肛管而非肛周，且表现为鳞状和基底样混合外观。两组在患者年龄、相邻发育异常的存在、导管分化或预后方面未发现差异。湿疣样肿瘤形态与HPV 16 DNA阳性之间无相关性；因此，无法鉴定出与外阴疣状癌相当的亚组。
