Lin T M, Shi G Y, Tsai C F, Su H J, Guo Y L, Wu H L
Department of Medical Technology, Medical College, National Cheng Kung University, Tainan, Taiwan, ROC.
J Virol Methods. 1997 Jan;63(1-2):219-25. doi: 10.1016/s0166-0934(96)02132-5.
The sensitivity of two different types of cells to bovine herpesvirus type 4 (BHV-4) was compared by median tissue culture infectious dose (TCID50) assays. The bovine arterial endothelial (BAE) cell culture derived from bovine carotid arteries was 100-1000 times more sensitive to two strains of BHV-4, Movar 33/63 and DN 599, than Madin Darby bovine kidney (MDBK) cell line commonly used for the propagation of these viruses. BAE cell cultures infected with BHV-4 displayed cytopathic effects (CPE) earlier and more prominently than the MDBK cells infected with the same viruses. BAE cells were also more sensitive than MDBK cells in conventional plaque assays in that the former developed well characterized and easily recognized plaques after infection with the viruses. BAE cells, which are proved to be exceptionally susceptible to BHV-4, can be used in the detection and quantitation of BHV-4.
通过半数组织培养感染剂量（TCID50）测定法比较了两种不同类型细胞对牛疱疹病毒4型（BHV-4）的敏感性。源自牛颈动脉的牛动脉内皮（BAE）细胞培养物对两种BHV-4毒株Movar 33/63和DN 599的敏感性比常用于这些病毒增殖的马-达二氏牛肾（MDBK）细胞系高100 - 1000倍。感染BHV-4的BAE细胞培养物比感染相同病毒的MDBK细胞更早且更明显地表现出细胞病变效应（CPE）。在传统的蚀斑测定中，BAE细胞也比MDBK细胞更敏感，因为前者在感染病毒后形成特征明显且易于识别的蚀斑。已证明对BHV-4异常敏感的BAE细胞可用于BHV-4的检测和定量。
