Kariuki S, Muthotho N, Kimari J, Waiyaki P, Hart C A, Gilks C F
Department of Medical Microbiology and Genito-Urinary Medicine, University of Liverpool, UK.
Trans R Soc Trop Med Hyg. 1996 Nov-Dec;90(6):712-4. doi: 10.1016/s0035-9203(96)90447-2.
Recently, an outbreak of dysentery due to multi-drug resistant Shigella dysenteriae type 1 strains was reported along the coastal area of Kenya and shortly thereafter another outbreak appeared in the outskirts of Nairobi. We analysed 22 multi-drug resistant S. Dysenteriae type 1 strains isolated from cases in the latter outbreak using plasmid deoxyribonucleic acid (DNA) profiles and pulse-field gel electrophoresis of genomic DNA. All isolates were resistant to commonly available drugs including ampicillin, trimethoprim, sulphamethoxazole, chloramphenicol, tetracycline and streptomycin with minimum inhibitory concentrations > 64 micrograms/mL, but were fully sensitive to gentamicin. Only 2 strains were resistant to nalidixic acid. Analysis of plasmid DNA and genomic DNA revealed that all 22 strains were clonally related. It is likely that the present outbreak was related to that on the coast, as suggested by the similarity in drug susceptibility data. The drug susceptibility and molecular epidemiological data provide a useful baseline for future monitoring of epidemic and endemic S. dysenteriae activity in East Africa.
最近，肯尼亚沿海地区报告了由多重耐药性痢疾志贺菌1型菌株引起的痢疾疫情，此后不久，内罗毕郊区也出现了另一起疫情。我们使用质粒脱氧核糖核酸（DNA）图谱和基因组DNA的脉冲场凝胶电泳分析了从后一次疫情病例中分离出的22株多重耐药性痢疾志贺菌1型菌株。所有分离株对常用药物耐药，包括氨苄青霉素、甲氧苄啶、磺胺甲恶唑、氯霉素、四环素和链霉素，最低抑菌浓度>64微克/毫升，但对庆大霉素完全敏感。只有2株对萘啶酸耐药。质粒DNA和基因组DNA分析表明，所有22株菌株在克隆上相关。正如药敏数据的相似性所表明的，目前的疫情很可能与沿海地区的疫情有关。药敏和分子流行病学数据为未来监测东非痢疾志贺菌的流行和地方病活动提供了有用的基线。
